Synthetic heparin pentasaccharides

ABSTRACT

Preparation of synthetic monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides for use in the preparation of synthetic heparinoids.

FIELD OF THE INVENTION

This invention is directed to intermediates, and a processes for the chemical synthesis of AT-III binding heparin or heparinoid, pentasaccharides.

BACKGROUND ART

Vascular thrombosis is a cardiovascular disease indicated by the partial or total occlusion of a blood vessel by a clot containing blood cells and fibrin. In arteries, it results predominantly from platelet activation and leads to heart attack, angina or stroke, whereas venous thrombosis results in inflammation and pulmonary emboli. The coagulation of blood is the result of a cascade of events employing various enzymes collectively known as activated blood-coagulation factors. Heparin, a powerful anticoagulant has been used since the late 1930's in the treatment of thrombosis. In its original implementation, tolerance problems were noted and so reduced dosage was suggested to reduce bleeding and improve efficacy. In the early 1970's, clinical trials did indeed indicate acceptable tolerance was obtainable whilst still preserving antithrombotic activity. Unfractioned heparin (UFH) is primarily used as an anticoagulant for both therapeutic and surgical indications, and is usually derived from either bovine lung or porcine mucosa. Amongst the modern uses of unfractioned heparin are the management of unstable angina, an adjunct to chemotherapy and anti-inflammatory treatment, and as a modulation agent for growth factors and treatment of haemodynamic disorders.

In the late 1980's, the development of low molecular weight heparins (LMWHs) led to improvements in antithrombotic therapy. LMWHs are derived from UFH by such processes as; chemical degradation, enzymatic depolymerisation and γ-radiation cleavage. This class of heparins have recently been used for treatment of trauma related thrombosis. Of particular interest is the fact that their relative effects on platelets are minimal compared to heparin, providing an immediate advantage when treating platelet compromised patients. The degree of depolymerisation of UFH can be controlled to obtain LMWH of different lengths. Dosage requirements for the treatment of deep vein thrombosis (DVT) are significantly reduced when employing LMWH as opposed to UFH, although in general the efficacy of both therapeutics seems to be comparable. In addition, LMWH can be effective as an alternative therapeutic for patients who have developed a sensitivity to UFH. Unfortunately, there has recently been a great deal of concern in the use of LMWH due to the perceived potential for cross-species viral contamination as a result of the animal source of the parent UFH.

One way of avoiding the possibility of cross-species contamination, is to prepare heparins by chemical synthesis. This method would also provide the opportunity to develop second generation heparins or heparinoids, that can be tailored to target particular biological events in the blood coagulation cascade.

An investigation to determine the critical structural motif required for an important binding event in a coagulation cascade involving heparin, dates back to the 1970's. Some structural features of heparin were defined, but the binding domains of interest remained essentially undefined. Research conducted by Lindahl and co-workers¹ and separately by Choay and co-workers² eventually led to the determination that a pentasaccharide sequence constituted the critical binding domain for the pro-anticoagulant cofactor, antithrombin III (AT-III). After determination of the critical heparin sugar sequence, complete chemical syntheses were embarked upon to further prove the theories. Complete syntheses of the pentasaccharide binding domain were completed at similar times by Sinay and co-workers³ and by Van Boeckel and co-workers⁴.

Significant difficulties were encountered during both these reported syntheses. The synthesis by Van Boeckel and co-workers provided a method on reasonable scale (156 mg's of final product) and with improved yields compared to the Sinay synthesis, but still only provided an overall yield of 0.22%, (compared with 0.053% for the Sinay synthesis). One particular problem encountered during the final deprotection, was the intermolecular reaction of the hemiacetal (the reducing end functionality of the sugar), which led to the formation dimers and trimers. To reduce the likelihood of this occuring, an α-methyl glycoside of the pentasaccharide was synthesised. The structures of interest are represented in FIG. 1, wherein I represents the hemiacetal form, and II represents the α-methylglycoside form.

As mentioned, studies have determined that the significant biological event in preventing thrombosis is the binding of a pentasaccharide sequence⁵ of heparin, to heparin cofactor antithrombin III (AT-III). As well as pentasaccharide I, the important derivative III has also been prepared by total synthesis⁶. Compound II has recently completed phase III clinical trials for the treatment of deep-vein thrombosis. The following patents display some relevance to the present invention. Patent U.S. Pat. No. 4,401,662 claims composition of matter on the pentasaccharide AT-III binding sequence of heparin as does U.S. Pat. No. 4,496,550. Patents EP 0,084,999 and U.S. Pat. No. 4,818,816 detail synthetic methodologies towards pentasaccharide I, and derivative II.

OBJECT OF THE INVENTION

It is an object of the invention to provide a synthetic preparation for heparin pentasaccharides, and intermediates thereof, and to novel intermediates for heparin pentasaccharides, and to novel heparin pentasaccharides.

The present invention provides composition of matter of intermediates, and a process for the synthesis, of AT-III binding heparins and heparinoids. What this entails is a stepwise synthetic process employing monosaccharide building blocks.

The nature of the AT-III binding pentasaccharide is such, that under cursory analysis of the individual monomeric units constituting the pentasaccharide, we note that each is distinct from the others. Secondly, we can see that there is an alternating stereospecificity in regard to the glycosidic linkages (FIG. 1).

In a synthesis, the difference evident in each block requires that each individual monomer used in the synthesis will need a different protecting group pattern. In light of this, it is essential in the synthesis of the above pentasaccharide that a protecting group strategy is carefully conceived. As can be seen, the pentasaccharide displays O-sulphation, N-sulphation, there are free hydroxyl groups, and there are stereospecific glycosidic linkages.

Therefore, a protection strategy is required such that (1) sulphation can be effected at the required sites, whilst leaving some hydroxyl groups unsulphated (note that due to the chemical lability of N- and O-sulphates, sulphation needs to be effected late in the synthesis), (2) a protection strategy is required that assists in effecting the appropriate glycosidic linkage and (3) a protection strategy is required that enables the correct (in terms of regio- and stereoisomerism) glycosidic linkages to be formed. α-Glycosidic linkages are typically generated by the use of what are known as non-participating protecting groups, whilst β-linkages are effected by participating protecting groups. Some N- and O-participating and nonparticipating protecting groups are known to the art (the art being considered carbohydrate chemistry). It is also well known to the art that the kind of protecting groups employed can effect the reactivity of the building block. The culmination of these requirements are demonstrated in the exemplary building block in FIG. 2 below, which displays the kind of characteristics required to effect the synthesis of heparin oligosaccharides.

In exemplary building block C, X is a leaving group suitable of reacting with another monomer or acceptor, to form an interglycosidic linkage; R¹ is a nonparticipating amino protecting group so as to effect an α-linkage upon activation of X followed by coupling to an appropriate acceptor; R² and R⁴ can be similarly protected to allow for eventual O-sulphation, whilst R³ is required to be differentially protected so as to allow the formation of an acceptor hydroxyl group to couple this block to the next in the chain. The building blocks in FIG. 3 exemplify the kind of derivatised monosaccharides required to effect the synthesis of heparin AT-III binding pentasaccharides.

The protecting groups represented by ‘R_(S)’ in FIG. 3 are sites that will eventually require O-sulphation, the protecting groups represented by ‘R_(H)’ need to be orthogonal to ‘R_(S)’ and represents sites that will eventually become hydroxyl groups. The substituents ‘X₁’ and ‘X₂’ represent leaving groups that are activated to react with another suitable protected building block to form a glycosidic linkage, and, in the case of X₁, may also be derivatised as alkyl glycosides or substituted with a group suitable to allow conjugation to a support for drug delivery. The ‘R_(L)’ groups are protecting groups orthogonal to both ‘R_(S)’ and ‘R_(H)’, and represent sites through which chain elongation via glycosylation occurs. ‘R’ is representative of either a protected or latent carboxylate function. The ‘R_(A)’ groups are non-participating amino protecting groups that enable α-linkages to be formed while the ‘R_(B)’ groups may be either a participating or nonparticipating amino protecting group. There is another level of complexity to be added to the synthesis in as much as the protecting groups in blocks D and B that are indicated by the boxes, need to be such that they allow for the formation of a β-glycosidic linkage. This may require a two stage protection at the indicated sites, ie. a protection followed by deprotection and subsequent reprotection with a different protecting group. The initial protection is required to effect the correct stereochemistry in a glycosylation, and second stage protection to allow for the correct sulphation pattern.

As is evident, the pentasaccharide can be constructed in a variety of different ways; blocks B and A can be coupled, blocks E and D can be coupled, block C can be coupled to either, and the resulting dimer and trimer can finally be coupled to form the pentasaccharide. Alternatively, each block can be added sequentially and so on. There are a number of alternative coupling sequences that can be easily conceived and the choice made in regard to this, in itself, has a marked effect on the synthetic methodologies that will finally be employed, and therefore impacts on the overall success of the synthesis.

In one aspect the invention provides for a monosaccharide building block in the D-glucopyrano configuration, for the preparation of synthetic heparinoids, said building block of General Formula I,

Wherein X₁ includes but is not limited to: hydroxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, or other suitable leaving group; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; other suitable groups will be known to those skilled in the art,

-   R_(A) includes but is not limited to: an azido function, an amine;     an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Cbz, NH-Troc, N-phthalimido;     or, other such suitable protected amino functions known to those     skilled in the art, -   R_(H) is a benzyl or substituted benzyl protecting group,

R_(L) includes but is not limited to: a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; or, other such suitable protecting groups as known to those skilled in the art, and

-   R_(S) includes but is not limited to: 4-methoxyphenyl; substituted     benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted     alkylacyl, arylacyl or alkylarylacy protecting groups; carbonate     protecting groups; or, other suitable protecting groups as known to     those skilled in the art.

Alternatively R_(L) and R_(S) can combine to form a benzylidene or substituted benzylidene ring.

In a second aspect the invention provides for a monosaccharide building block in the L-idopyrano conformation, for the preparation of synthetic heparinoids, said building block of General Formula II,

Wherein X₂ includes but is not limited to: a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, or other suitable leaving group; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; other suitable groups will be known to those skilled in the art,

-   R_(S) is defined as in General Formula I, -   R_(H) is defined as in General Formula I, -   R_(L) is defined as in General Formula I, and -   R_(E) includes but is not limited to: methyl, C₂-C₅ alkyl;     substituted alkyl; or, benzyl and substituted benzyl groups; other     suitable groups will be known to those skilled in the art.

In a third aspect the invention provides for a monosaccharide building block in the L-idopyrano configuration, for the preparation of synthetic heparinoids, said building block of General Formula III,

Wherein X₂ is defined as in General Formula II,

-   R_(S) is defined as in General Formula II, -   R_(H) is defined as in General Formula I, -   R_(L) is defined as in General Formula I, and -   R_(M) includes but is not limited to a p-methoxyphenyl protecting     group or other suitable oxidatively labile protecting group; a     trityl group; or, other such suitable protecting groups as known to     those skilled in the art.

In a fourth aspect the invention provides for a monosaccharide building block in the D-glucopyrano configuration for the preparation of synthetic heparinoids, said building block of General Formula IV,

Wherein X₂ is defined as in General Formula II,

-   R_(B) includes but is not limited to: an azido function, an amine;     an NH-Dde or NH-DTPM group; or other suitably protected amino     functions as known to those skilled in the art, -   R_(L) is defined as in General Formula I, and -   R_(S) is defined as in General Formula I.

In a fifth aspect the invention provides for a monosaccharide building block in the D-glucuronate configuration for the preparation of synthetic heparinoids, said building block of General Formula V,

Wherein X₂ is as defined in General Formula II,

-   R_(P) includes but is not limited to: 4-methoxyphenyl; substituted     benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted     alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate     protecting groups; or, other suitable protecting groups as known to     those skilled in the art. -   R_(L) is defined as in General Formula I, and -   R_(E) is defined as in General Formula II.

In a sixth aspect the invention provides for a monosaccharide building block in the D-glucopyrano configuration for the preparation of synthetic heparinoids, said building block of General Formula VI,

Wherein X₂ is as defined as in General Formula II,

-   R_(B) is defined as in General Formula IV, -   R_(H) may be selected independently and are defined as in General     Formula I, and -   R_(S) is defined as in General Formula I.

In a seventh aspect the invention provides for a monosaccharide building block in the D-glucopyrano configuration for the preparation of synthetic heparinoids, said building block of General Formula VII,

Wherein X₁ is defined as in General Formula I,

-   R_(L) is defined as in General Formula I, and -   R_(S) is defined as in General Formula I. -   R_(L) and R_(S) may also together combine to form a benzylidene or     substituted benzylidene ring.

In an eighth aspect the invention provides for a disaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula VIII,

Wherein X₁ is defined as in General Formula I,

-   R_(H1) is defined as being selected from R_(H) of General Formula I,     with the addition that R_(H1) and R_(A) can combine together to form     a cyclic carbamate, -   R_(A) is defined as in General Formula I, with the addition that     R_(H1) and R_(A) can combine together to form a cyclic carbamate -   R_(S) is defined as in General Formula I, -   R_(H) is defined as in General Formula I, -   R_(L) is defined as in General Formula I, and -   R_(E) is defined as in General Formula II.

In a ninth aspect the invention provides for a disaccharide building block for the preparation of synthetic heparinoids, said building block of General

Wherein X₁ is as defined as in General Formula I,

-   R_(A) is defined as in General Formula XIII, -   R_(H1) is defined as in General Formula XIII, -   R_(S) is defined as in General Formula I, -   R_(L) is defined as in General Formula I, and -   R_(M) is defined as in General Formula III.

In a tenth aspect the invention provides for a disaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula X,

Wherein X₂ is as defined in General Formula II,

-   R_(S1) is defined as being selected from R_(S) of General Formula I,     with the addition that R_(S1) and R_(B) can combine together to form     a cyclic carbamate. -   R_(B) is defined as in General Formula IV, with the addition that     R_(S1) and R_(B) can combine together to form a cyclic carbamate. -   R_(S) is defined as in General Formula I, -   R_(P) are defined as in General Formula V, -   R_(L) is defined as in General Formula I, and -   R_(E) is defined as in General Formula II.

In an eleventh aspect the invention provides for a disaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula XI,

Wherein X₂ is as defined in General Formula II,

-   R_(P) are defined as in General Formula V, -   R_(E) is defined as in General Formula II, -   R_(B) is defined as in General Formula IV, with the addition the     R_(B) and R_(H2) can combine to form a cyclic carbamate, -   R_(H2) is defined as being selected from R_(H) of General Formula I,     with the addition that R_(B) and R_(H2) can combine to form a cyclic     carbamate, -   R_(H) is defined as in General Formula I, and -   R_(S) is defined as in General Formula I.

In a twelfth aspect the invention provides for a disaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula XII,

Wherein X₂ is as defined in General Formula II,

-   R_(P) are defined as in General Formula V, -   R_(M) is defined as in General Formula III, -   R_(B) and R_(H2) are as defined in General Formula XI, -   R_(H) is defined as in General Formula I, and -   R_(S) is defined as in General Formula I.

In a thirteenth aspect the invention provides for a disaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula XIII,

Wherein X₂ is defined as in General Formula II,

-   R_(B) and R_(S1) are defined as in General Formula X, -   R_(S) is defined as in General Formula I, -   R_(P) is defined as in General Formula V, and -   A includes but is not limited to; H, Methoxy, Methyl; other suitable     substituents will be known to those in the art.

In a fourteenth aspect the invention provides for a trisaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula XIV,

Wherein X₂ is defined as in General Formula II,

-   R_(B) and R_(S1) are defined as in General Formula X, -   R_(S) is defined as in General Formula I, -   R_(P) is defined as in General Formula V, -   R_(E) is defined as in General Formula II, -   R_(B1) is defined as being selected from R_(B) of General Formula     IV, with the addition that R_(B1) can combine together with R_(H2)     to form a cyclic carbamate, -   R_(H2) is defined as being selected from R_(H) of General Formula I,     with the addition that R_(H2) can combine together with R_(B1) to     form a cyclic carbamate, and -   R_(H) is defined as in General Formula I.

In a fifteenth aspect the invention provides for a trisaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula XV,

Wherein X₁ is defined as in General Formula I

-   R_(A) and R_(H1) are defined as in General Formula VIII, -   R_(S) is defined as in General Formula I, -   R_(H) is defined as in General Formula I, -   R_(E) is defined as in General Formula II, -   R_(B) and R_(S1) are defined as in General Formula X, and -   R_(L) is defined as in General Formula I.

In a sixteenth aspect the invention provides for a tetrasaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula XVI,

Wherein X₁ is defined as in General Formula I,

-   R_(A) and R_(H1) are defined as in General Formula VIII, -   R_(S) is defined as in General Formula I, -   R_(H) is defined as in General Formula I, -   R_(E) is defined as in General Formula II, -   R_(B) and R_(S1) are defined as in General Formula X, -   R_(P) is as defined in General Formula V, and -   R_(L) is as defined in General Formula I.

In an seventeenth aspect the invention provides for a tetrasaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula XVII,

Wherein X₂ is defined as in General Formula IV,

-   R_(H) is defined as in General Formula I, -   R_(E) is defined as in General Formula II, -   R_(B) and R_(S1) are defined as in General Formula X, -   R_(S) is defined as in General Formula I, -   R_(P) is defined as in General Formula V, -   R_(L) is defined as in General Formula I, and -   R_(B1) and R_(H2) are defined as in General Formula XIV.

In a eighteenth aspect the invention provides for a pentasaccharide building block for the preparation of synthetic heparinoids, said building block of General Formula XIII,

Wherein X₁ is defined as in General Formula I

-   R_(A) and R_(H1) are defined as in General Formula VIII, -   R_(S) is defined as in General Formula I, -   R_(H) is defined as in General Formula I, -   R_(E) is defined as in General Formula II, -   R_(B) and R_(S1) are defined as in General Formula X, -   R_(P) is defined as in General Formula V, and -   R_(B1) and R_(H2) are defined as in General Formula XIV.

In a nineteenth aspect, the invention provides a method for the preparation of compounds of the eighth aspect, involving the step of reacting a compound of the second or third aspect with a compound of the first or seventh aspect to form a new glycosidic bond.

In a twentieth aspect, the invention provides a method for the preparation of compounds of the eighth aspect, involving the step of selectively removing the protecting group R_(M) from compounds of the ninth aspect and oxidizing the product of said deprotection.

In a twenty first aspect, the invention provides a method for the preparation of compounds of the tenth aspect, involving the step of reacting a compound of the fifth aspect with a compound of the fourth or seventh aspect to form a new glycosidic bond.

In a twenty second aspect, the invention provides a method for the preparation of compounds of the eleventh aspect, involving the step of reacting a compound of the fifth aspect with a compound of the sixth or seventh aspect to form a new glycosidic bond.

In a twenty third aspect, the invention provides a method for preparation of compounds of the thirteenth aspect involving the reaction of a compound of the fourth or seventh aspect with a suitable donor molecule, to form a new glycosidic bond.

In a twenty fourth aspect, the invention provides a method for the preparation of compounds of the fourteenth aspect involving the step of using any one or more of the compounds of the fourth, fifth, sixth, seventh, tenth, eleventh, twelfth or thirteenth aspect in a glycosidic bond forming reaction.

In a twenty fifth aspect, the invention provides a method for the preparation of compounds of the fifteenth aspect involving the step of using any one or more compounds of the first, second, third, fourth, seventh, eighth and ninth aspects in a glycosidic bond forming reaction.

In a twenty sixth aspect, the invention provides a method for the preparation of compounds of the sixteenth aspect involving the step of using any one or more of the compounds of the first, second third, fourth, fifth, seventh, eighth, ninth, tenth, thirteenth or fifteenth aspect in a glycosidic bond forming reaction.

In a twenty seventh aspect, the invention provides a method for the preparation of compounds of the seventeenth aspect involving the step of using any one or more of the compounds of the second, third, fourth, fifth, seventh, tenth, eleventh, twelth, thirteenth or fourteenth aspect in a glycosidic bond forming reaction.

In a twenty eighth aspect, the invention provides a method for the preparation of compounds of the eighteenth aspect involving the step of using any one or more of the compounds of the 1,2,3,4, 5, 7, 8, 9, 10, 11, 12, 13, 14, or 15, 16 or 17^(th) aspect in a glycosidic bond forming reaction.

Best Mode

Embodiments of the Invention will be Described with Reference to the Following Examples: Standard Operating Protocols are Provided for Many of the Examples.

List of Abbrevations:

-   AcO: Acetyl, -   All: Allyl, -   Alloc: Allyloxycarbonyl, -   Bn: Benzyl, -   Bz: Benzoyl, -   CAN: (NH₄)₂Ce^(IV)(NO₃)₆, ceric ammonium (IV) nitrate, -   ClAc: Monochloroacetyl, -   Cres: p-Tolyl, -   DCC: Dicyclohexylcarbodiimide, -   Dde: 1-(4,4-dimethyl-2,6-dioxocyclohex-ylidene)ethyl, -   DEAD: Diethyl azodicarboxylate, -   DIPEA: Diisopropylethylamine, -   DMAP: 4-N,N-dimethylaminopyridine, -   DMF: N,N-Dimethylformamide, -   DMTST: Dimethyl(methylthio)sulfoniumtetrafluoromethansulfonate, -   DTPM: (1,3-dimethyl-2,4,6 (1H, 3H,     5H)-trioxopyrimidin-5-ylidene)methyl, -   Lev: 4-Oxopentanoyl, -   MCPBA: 3-chloroperbenzoic acid, -   Mes: Methanesulfonyl, -   Mp: 4-Methoxyphenyl, -   Mpm: 4-methoxybenzyl, -   NBS: N-Bromosuccinimide, -   NIS: N-Iodosuccinimide, -   NMP: N-Methylpyrollidone -   NPht: N-Phthaloyl -   PDC: Pyridiniumdichromate, -   Pent: n-Pentenyl, -   Ph₃P: Triphenylphosphine, -   Piv: Pivaloyl, -   TBAF: Tetrabutylammoniumfluoride, -   TBDMS: tert-Butyldimethylsilyl, -   TBDPS: tert-Butyldiphenylsilyl, -   TCA: Trichloroacetimidyl, -   TEMPO: 2,2,6,6-Tetramethyl-1-piperidinyloxyl, -   TFA: Trifluoroacetic acid, -   TFAA: Trifluoroacetic acid anhydride, -   Tf: Trifluoromethanesulfonyl, -   TfN₃: Trifluoromethanesulfonyl azide, prepared from NaN₃ and Tf₂O, -   TfOH: Trifluoromethanesulfonic acid, -   THF: Terahydrofuran, -   TMS: Trimethylsilyl, -   Tos: p-Toluenesulfonyl, -   p-TosOH: p-Toluenesulfonic acid, -   Trit: Triphenylmethyl.     Standard Operating Procedures -   Standard Operating Procedure 1: Formation of Benzylidene acetals -   Standard Operating Procedure 2: Formation of p-Methoxybenzylidene     acetals -   Standard Operating Procedure 3: Formation of isopropylidene acetals: -   Standard Operating Procedure 4: Dealkylidenation (Removal of     isopropylidene, benzylidene and p-methoxybenzylidene) -   Standard Operating Procedure 5: Regioselective opening of the     p-methoxybenzyliden acetal to a 6-O-pMethoxybenzyl ether -   Standard Operating Procedure 6: Regioselective opening of a     benzylidene ring to a 4-O-benzyl ether -   Standard Operating Procedure 7: Introduction of a benzyl or     p-methoxybenzyl ether -   Standard Operating Procedure 8: Introdution of a     tert-butyldiphenylsilyl ether -   Standard Operating Procedure 9: Cleavage of a     tert-Butyldiphenylsilyl ether -   Standard Operating Procedure 10: Introduction of a N-DTPM-group -   Standard Operating Procedure 11: Cleavage of a N-DTPM-group -   Standard Operating Procedure 12: Introduction of an azide group via     diazo transfer reaction -   Standard Operating Procedure 13: Hydrolysis of thioglycosides (NBS) -   Standard Operating Procedure 14: Hydrolysis of thioglycosides (NIS) -   Standard Operating Procedure 15: Chemoselective Oxidation to Uronic     acids -   Standard Operating Procedure 16: Methyl ester formation on the     Uronic acids -   Standard Operating Procedure 17: Regioselective 6-O-Benzoylation -   Standard Operating Procedure 18: Common procedure for O-Benzoylation -   Standard Operating Procedure 19: Common procedure for O-Acetylation -   Standard Operating Procedure 20: PDC-oxidation of alcohols to     carboxylic acids -   Standard Operating Procedure 21: Chemoselective 1-O-Benzoyl cleavage -   Standard Operating Procedure 22: Deacylation under Zemplen     conditions -   Standard Operating Procedure 23: Introduction of the 4-Oxopentanoyl     (=Levulinoyl) group -   Standard Operating Procedure 24: Cleavage of the 4-Oxopentanoyl     (=Levulinoyl) group -   Standard Operating Procedure 25: Formation of Trichloroacetimidates -   Standard Operating Procedure 26: Regioselective introduction of a     6-O-p-Methoxyphenyl group under Mitsunobu conditions -   Standard Operating Procedure 27: Cleavage of the p-Methoxyphenyl     ether -   Standard Operating Procedure 28: Cleavage of p-Methoxybenzyl ethers -   Standard Operating Procedure 29: Formation of a 2,3-cyclic carbamate -   Standard Operating Procedure 30: Cleavage of the N-phthaloyl group -   Standard Operating Procedure 31: Introduction of a thiocresyl ether     at the reducing end -   Standard Operating Procedure 32: Glycosylation with thioglycosides -   a) NIS-promoted glycosylation -   b) DMTST promoted glycosylations: -   Standard Operating Procedure 33: Glycosylations with     trichloroacetimidates -   Standard Operating Procedure 34: Glycosylations using     2,3-cyclocarbamoyl protected pThiocresyl glycosides as glycosyl     donors -   Standard Operating Procedure 35: Introduction of an Alloc-group -   Standard Operating Procedure 36: Cleavage of an Alloc-group -   Standard Operating Procedure 37: Lewis acid mediated benzylation -   Standard Operating Procedure 38: benzylation under mild basic     conditions -   Standard Operating Procedure 39: Ester cleavage under very mild     conditions     Standard Operating Procedure 1: Formation of Benzylidene Acetals

The starting material (47.5 mmol) was dissolved in acetonitrile (100-200 ml) and reacted with benzaldehyde dimethyl acetal (1.2 equiv.) and a catalytic amount of p-toluenesulphonic acid monohydrate (0.01-0.1 equiv). The reaction was stirred at 50° C. under reduced pressure (350 mbar) until the TLC shows completion. Subsequently, the mixture was neutralized with triethylamine (pH≈9) and concentrated in vacuo. The remaining residue was dissolved in an organic solvent (e.g. dichloromethane or ethyl acetate) and extracted with H₂O, saturated brine solution, dried over Na₂SO₄ and concentrated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 70 and 95%.

Standard Operating Procedure 2: Formation of p-Methoxybenzylidene acetals

The starting material (47.5 mmol) was dissolved in DMF/acetonitrile (1/1, 100-200 ml) and reacted with p-methoxybenzaldehyde dimethyl acetal (1.2 equiv.) and a catalytic amount of p-toluenesulphonic acid monohydrate (0.01-0.1 equiv). The reaction was stirred between 50-60° C. under reduced pressure (350 mbar) until the TLC shows completion. Subsequently, the mixture was neutralized with triethylamine (pH≈9) and concentrated in vacuo. The remaining residue was dissolved in an organic solvent (e.g. dichloromethane or ethyl acetate) and extracted with H₂O, saturated brine solution, dried over Na₂SO₄ and concentrated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 70 and 85%.

Standard Operating Procedure 3: Formation of Isopropylidene Acetals:

A solution of starting material (10 mmol) and catalytic amounts of camphorsulfonic acid (0.01-0.1 equiv) in 2,2-dimethoxypropane (50 ml) was stirred at 25° C. until completion, neutralized with triethylamine and concentrated. The remaining residue was dissolved in an organic solvent (e.g. dichloromethane or ethyl acetate) and extracted with H₂O and saturated brine solution. The organic layer was dried over Na₂SO₄ and concentrated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 75 and 93%.

Standard Operating Procedure 4:

Dealkylidenation (Removal of Isopropylidene, Benzylidene and p-methoxybenzylidene)

A solution of the acetal (31 mmol) in 150 ml dichloromethane was cooled to 0° C. and reacted with 80% aqueous TFA (20.0 ml, cooled to 0° C.). After stirring at 0° C. until completion, the reaction mixture was neutralized with 30% NaOH solution and extracted with water and saturated brine solution. The organic layer was dried over Na₂SO₄ and concentrated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 70 and 95%.

Modification Using p-TosOHxOH₂ in MeOH/CH₃CN for Cleavage:

The acetal (16.6 mmol) was dissolved in 100 mL of dry acetonitrile and 25 mL MeOH and the solution was reacted with catalytic amounts of p-TosOHxOH₂. The reaction mixture was heated at elevated Temperature (between 40 and 60° C.) until completion and then neutralized with Et₃N, concentrated in vacuo and purified either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 70 and 95%.

Standard Operating Procedure 5: Regioselective Opening of the p-Methoxybenzyliden Acetal to a 6-O-pMethoxybenzyl Ether

A suspension of the starting sugar (10.2 mmol), molecular sieves 3 Å (6.5 g, freshly activated) and Na(CN)BH₃ (3.85 g, 61.2 mmol) in dry DMF (90 ml) was stirred for 1 hr at r.t. and cooled down to 0° C. Subsequently, a solution of TFA (11.2 mL, 122.4 mmol in 51 mL dry DMF) was added dropwise and stirring continued at 50 to 60° C. until completion of the reaction. The reaction mixture was cooled to 20° C., diluted with ethyl acetate and extracted with a saturated aqueous NaHCO₃ solution and filtered through a celite pad. The combined organic layers were washed with saturated brine solution, dried over MgSO₄ and concentrated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 70 and 90%.

Standard Operating Procedure 6: Regioselective Opening of a Benzylidene Ring to a 4-O-benzyl Ether

A solution of the starting material (3.4 mmol) in 25 mL dichloromethane is cooled to 0° C. and to it is added of a solution of BH₃ in THF (1 M, 34 ml) and a solution of Bu₂BOTf in dichloromethane (1 M, 3.7 ml). The reaction is stirred at 0° C. till completion and then quenched with 10 ml Et₃N and 10 ml MeOH, concentrated and coevaporated three times with toluene. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yield for the product formation varied between 75 and 90%.

Standard Operating Procedure 7: Introduction of a Benzyl or p-methoxybenzyl Ether

The starting material (40.2 mmol) was dissolved in dry N,N′-dimethylformamide (100 mL) at 0° C. and reacted with NaH (48.24 mmol, 1.2 eq. per OH to be benzylated). Then benzyl bromide (1.1 eq per OH to be benzylated) was added dropwise and stirring continued at 0° C. until completion. The same conditions were applied for the introduction of an allyl ether (Allylbromide served as allylating reagent).

The excess of NaH was neutralized by careful addition of acetic acid, followed by concentration of the reaction mixture in vacuo. The residue was dissolved in ethyl acetate and subsequently washed with water, 10% aqueous HCl solution, saturated aqueous NaHCO₃ solution, saturated brine solution, dried over Na₂SO₄ and concentrated in vacuo. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yield for the product formation varied between 70 and 92%.

The same procedure was followed for the formation of the p-methoxybenzyl ether except that p-methoxybenzyl chloride was added to the reaction instead of benzyl bromide and the reaction was performed between 50 and 60° C.

Standard Operating Procedure 8:

Introdution of a Tert-Butyldiphenylsilyl Ether

A mixture of the starting material (29.0 mmol) and imidazole (70.1 mmol) was dissolved in 80 mL anhydrous DMF and heated to 55° C. To the solution was added tert-butyldiphenylchlorosilane (8.30 mL, 31.9 mmol) and stirring continued at 55° C. until completion. The reaction mixture was then cooled to 20° C. and quenched with aqueous NaHCO₃ solution. After concentration in vacuo, the residue was taken up in ethyl acetate and the organic phase washed successively with water, 10% aqueous citric acid, water, saturated brine solution, dried over Na₂SO₄ and evaporated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 85 and 95%.

Standard Operating Procedure 9: Cleavage of a Tert-Butyl-diphenylsilyl Ether

To a solution of the silyl ether (2.15 mmol) in 2.5 mL dry THF and acetic acid (3.44 mmol) was added 1M TBAF solution in THF (3.22 mL) and stirring continued till completion of the reaction. Subsequently, the reaction mixture was concentrated in vacuo. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 85 and 97%.

Standard Operating Procedure 10: Introduction of a N-DTPM-Group

To a solution of the starting amine (24.5 mmol) in methanol (60 ml) is added a solution of the DTPM reagent (5.43 g, 25.7 mmol) in methanol (60 ml) at 60° C. After completion of the reaction, the reaction mixture was concentrated in vacuo, taken up in dichloromethane, extracted with water and saturated brine solution, dried over MgSO₄ and evaporated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 85 and 97%.

Standard Operating Procedure 11: Cleavage of a N-DTPM-Group

The starting material (40.94 mmol) was dissolved in dry DMF (50 ml) and reacted with ethylene diamine (20 ml) at room temperature until completion. The reaction mixture was concentrated in vacuo and coevaporated with toluene. The residue was suspended in CHCl₃ and filtered through a Celite pad. The filtrate was evaporated and final purification of the residue was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 85 and 92%.

Standard Operating Procedure 12: Introduction of an Azide Group via Diazo Transfer Reaction

a) Preparation of a Trifluoromethansulfonylazide Solution:

A solution of sodium azide (492 mmol) in water (80 mL) was prepared under N₂-atmosphere. To this stirred solution was added dichloromethane (100 mL) at 0° C., followed by the addition of triflic anhydride (16.5 ml) over 10 min. The mixture was further stirred for 2 hours at 0° C., the organic layer was separated and the aqueous layer was extracted with dichloromethane (2×40 mL). The combined organic layers were washed with saturated, aqueous NaHCO₃ solution (80 mL), water (80 mL) and dried over Na₂SO₄. After filtration, this solution was directly used for the diazotransfer reaction.

b) Diazotransfer Reaction:

To a solution of the starting material (26.0 mmol) and 4-N,N′-(dimethylamino)pyridine (14.5 g) in acetonitrile (100 mL) was added dropwise TfN₃-solution (85 ml) at room temperature within 10 min. The reaction was stirred till complete conversion of the starting material into the product. The reaction mixture was concentrated in vacuo to 30 ml and suspended in chloroform. After filtration through a Celite pad, the filtrate was concentrated and the residue was purified by filtration through a short silica gel pad. The typical yields for the product formation varied between 85 and 95%.

Standard Operating Procedure 13: Hydrolysis of Thioglycosides (NBS)

The starting thioglycoside (33.4 mmol) was suspended in 240 ml Acetone and 18 ml of distilled water and stirred for 45 min at −20° C. After addition of NBS (155 mmol) stirring was continued at −20° C. After completion, the reaction was stopped by addition of NaS₂O₃/NaHCO₃ (20% aqueous solution., 1/1) and the mixture diluted with ethyl acetate, subsequently washed with water and saturated brine solution. The organic layer was dried over Na₂SO₄ and concentrated in vacuo. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 75 and 90%.

Standard Operating Procedure 14: Hydrolysis of Thioglycosides (NIS)

The starting thioglycoside (33.4 mmol) was suspended in 240 ml Acetone and 18 ml of distilled water and stirred for 45 min at −20° C. After addition of NIS (56.8 mmol) and TMSOTf (2.84 mmol) stirring was continued until completion. The reaction was stopped by addition of NaS₂O₃/NaHCO₃ (20% aqueous solution, 1/1), diluted with ethyl acetate and washed with water and saturated brine solution. The organic layer was dried over Na₂SO₄ and concentrated in vacuo. Final purification was achieved either by crystallization (e.g. petroleum spirit/ethylacetate) or by silica gel chromatography. The typical yields for the product formation varied between 79 and 92%.

Standard Operating Procedure 15: Chemoselective Oxidation to Uronic Acids

A solution of the starting material (20.0 mmol) in dichloromethane (141 ml) was cooled to 0° C. and subsequently mixed with TEMPO (0.205 mmol in 12.8 ml dichloromethane), Aliquat 336 (12.8 ml of a 0.08 M solution in dichloromethane) and KBr (2.08 mmol in 4.17 ml H₂O) and stirring continued at 0° C. After 5 mins, a suspension of Ca(OCl)₂ (43.6 mmol) and NaHCO₃ (43.6 mmol) in 135 ml H₂O was added within 15 mins to the reaction mixture and stirring at 0° C. was continued till completion. The reaction was concentrated in vacuo and freeze dried. The crude residue was used as such for the next reactions.

Standard Operating Procedure 16: Methyl Ester Formation on the Uronic Acids

The crude residue of the oxidation to the uronic acid was dissolved in 50 ml Toluene and 50 ml Methanol and titurated with TMSCHN₂-solution (2M in hexane) until completion. The reaction mixture was quenched with acetic acid to destroy excess of esterification reagent and evaporated in vacuo. Final purification was achieved by silica gel chromatography. The typical yields for the product formation varied between 65 and 80% over the steps oxidation and esterification.

Standard Operating Procedure 17: Regioselective 6-O-Benzoylation

The starting material (32.04 mmol) was dissolved in dry dichloromethane (50 mL) and dry pyridine (10 mL) and cooled down to −45° C. Benzoyl chloride (32.04 mmol) was added dropwise and stirring continued at −45° C. till completion. The reaction was concentrated in vacuo and coevaporated with toluene three times. The remaining residue was dissolved in dichloromethane and washed with 10% aqueous citric acid solution, saturated aqueous NaHCO₃ solution and saturated brine solution, dried over Na₂SO₄ and evaporated in vacuo. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 75 and 94%.

Standard Operating Procedure 18: Common Procedure for O-Benzoylation

To a solution of the starting material (11.9 mmol) and DMAP (13.6 mmol) in 1,2-dichloroethane was added dropwise benzoylchloride (1.7 g, 12.1 mmol). at 0° C. The mixture was then left to stir until completion (dependent on the substrate between 20 to 55° C.). Subsequently, the reaction mixture was diluted with dichloromethane and washed with water, 5% NaHSO₄ solution, saturated aqueous NaHCO₃ solution and saturated brine solution. The organic layer was dried over MgSO₄ followed by removal of the solvent in vacuo to give a crude residue. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 80 and 96%.

Standard Operating Procedure 19: Common Procedure for O-Acetylation

To a suspension of the starting material (235 mmol, 3 acetylation sites) in pyridine (350 ml) at 0° C. was added dropwise acetic anhydride (175 ml). After completion of the addition, the reaction was allowed to return to room temperature and stirred until completion. The reaction mixture was evaporated to dryness and 3× coevaporated with toluene. The residue was taken up in dichloromethane and washed with 5% aqueous NaHSO₄-solution, saturated aqueous NaHCO₃-solution, water and saturated brine solution. The organic layer was dried over MgSO₄ and evaporated. Final purification of the residue was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 88 and 98%.

Standard Operating Procedure 20: PDC-Oxidation of Alcohols to Carboxylic Acids

The starting material (1.15 mol) was dissolved in anhydrous DMF (7.0 ml) and reacted with PDC (11.5 mmol) under stirring at room temperature until complete conversion into the uronic acid. The reaction mixture was subsequently poured into 50 ml water and the whole extracted with diethyl ether. The combined ether layers were washed with 10% aqueous citric acid solution, filtered through a short silica gel pad, dried over MgSO₄, evaporated and dried under high vacuum.

Standard Operating Procedure 21: Chemoselective 1-O-Benzoyl Cleavage

The starting material (36.8 mmol) was dissolved in dry DMF (80 ml) and cooled to 0° C. Subsequently, hydrazine acetate (44.06 mmol) was added and stirring continued until completion. After addition of acetone and acetic acid the reaction mixture was concentrated in vacuo. The residue was dissolved in dichloromethane and extracted with 10% aqueous citric acid solution, saturated NaHCO₃ solution, water and saturated brine solution, dried over MgSO₄, evaporated and dried under high vaccuum. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 72 and 88%.

Standard Operating Procedure 22: Deacylation Under Zemplen Conditions

The starting material (23.7 mmol) was suspended in dry MeOH (70 ml) and stirred for 30 mins at 0° C. Subsequently, NaOMe (0.1 equiv./O-Acyl group) was added (positive flush of N₂) and stirring was continued at 0° C. until completion. Finally, the reaction was neutralized with 10% aqueous HCl and the solvent evaporated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yield for the product formation varied between 90 and 98%.

Standard Operating Procedure 23: Introduction of the 4-Oxopentanoyl (=Levulinoyl) Group

a) Preparation of the Lev₂O Solution:

To a solution of DCC (31.2 mmol) in 100 mL dichloromethane was added levulinic acid (62.4 mmol) and DIPEA (62.42 mmol). The supernatant was used as such for the levulination reaction.

Reaction

The above Lev₂O solution was added to a solution of the starting sugar (15.6 mmol) dissolved in 25 mL of dry dichloromethane and stirring was continued until completion. Subsequently, the reaction mixture was filtered through a Celite pad and all combined organic layers were extracted with 10% aqueous citric acid solution, saturated aqueous brine solution, dried with Na₂SO₄ and concentrated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 85 and 96%.

Standard Operating Procedure 24: Cleavage of the 4-Oxopentanoyl (=Levulinoyl) Group

A solution of the starting sugar (1.28 mmol) and acetic acid (1.35 mL) in pyridine (5.0 mL) was cooled to 0° C. followed by addition of hydrazine hydrate (200 μL). Stirring at 0° C. was continued until completion and the reaction mixture diluted with dichloromethane, subsequently extracted with 10% aqueous citric acid, 10% aqueous NaHCO₃ solution, saturated brine solution, dried over Na₂SO₄, filtered and concentrated. Final purification was achieved either by crystallization or silica gel chromatography. The typical yields for the product formation varied between 80 and 95%.

Standard Operating Procedure 25: Formation of Trichloroacetimidates

a) with DBU:

A solution of the starting sugar (1.99 mmol) and trichloroacetonitrile (601 μL, 5.99 mmol) in 5 mL dry dichloromethane was stirred at room temperature for 30 min. The reaction mixture was then cooled to 0° C. and DBU (100 μmol) added. Stirring was continued until completion (dependent on the substrate, stirring was performed from 0° C. to 20° C.). The reaction mixture was concentrated to one half of its volume and directly loaded on a short plug of silica gel and purified via silica gel chromatography. The typical yields for the product formation varied between 78 and 95%.

b) With K₂CO₃:

A solution of the starting sugar (1.99 mmol) and trichloroacetonitrile (601 μL, 5.99 mmol) in 5 mL dry dichloromethane is stirred at rt for 30 min. The reaction mixture was then cooled down to 0° C. and anhydrous K₂CO₃ (19.9 mmol) added. The reaction was stirred at 0° C. till completion and then filtered through a celite pad. The filtrate was dried over Na₂SO₄ and evaporated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yield for the product formation varied between 78 and 95%.

Standard Operating Procedure 26: Regioselective Introduction of a 6-O-pMethoxyphenyl Group Under Mitsunobu Conditions

A solution of the starting sugar (13.52 mmol), 4-methoxyphenol (20.3 mmol) and triphenylphosphine (20.3 mmol) in 85 ml dry dichloromethane was stirred at 0° C. for 45 min. After addition of DEAD-reagent (22.9 mmol) at 0° C., the reaction mixture was further stirred at room temperature until completion, filtered through a celite pad, diluted with dichloromethane and extracted with 10% aqueous NaHCO₃/NaOH solution (1/1), 10% aqueous citric acid solution and aqueous saturated brine solution. The organic layer was dried over Na₂SO₄ and concentrated. Final purification was achieved by silica gel chromatography. The typical yield for the product formation varied between 70 and 89%.

Standard Operating Procedure 27: Cleavage of the P-Methoxyphenyl Ether

The starting material (1.18 mmol) was dissolved in 30 ml acetonitrile and 7.5 ml water and cooled to 0° C. Subsequently, CAN (3.83 mmol) was added and stirring continued at CPC until completion. The reaction mixture was diluted with ethyl acetate and extracted with water. The aqueous layer was made alkaline by addition of solid NaHCO₃ and back extracted with ethyl acetate. The combined organic layers were extracted with saturated aqueous NaHCO₃ solution and saturated brine solution, dried over MgSO₄ and evaporated. Final purification was achieved by silica gel chromatography. The typical yields for the product formation varied between 73 and 89%.

Standard Operating Procedure 28: Cleavage of p-Methoxybenzyl Ethers

The starting material (0.60 mmol) was dissolved in 27 ml acetonitrile and 3.0 ml water and cooled to 0° C. Subsequently, CAN (4.5 equiv.) was added and stirring continued from 0° C. to room temperature until completion. The reaction mixture was diluted with ethyl acetate and extracted with water. The aqueous layer was made alkaline by addition of solid NaHCO₃ and back extracted with ethyl acetate. The combined organic layers were extracted with saturated aqueous NaHCO₃ solution and saturated brine solution, dried over MgSO₄ and evaporated. Final purification was achieved by silica gel chromatography. The typical yields for the product formation varied between 73 and 85%.

Standard Operating Procedure 29: Formation of a 2,3-cyclic Carbamate

To a stirred solution of the starting material (3.56 mmol) in dichloromethane (100 ml) and 10% aqueous solution of NaHCO₃ (75 ml) was added a solution of triphosgene (1.25 mmol) in 10 ml dry dichloromethane. The reaction was stirred at room temperature till completion. The organic phase was washed with water, dried over Na₂SO₄, filtered and concentrated. Final purification was achieved either by crystallization or silica gel chromatography. The typical yield for the product formation varied between 75 and 95%.

Standard Operating Procedure 30: Cleavage of the N-phthaloyl Group

The N-phthaloylated starting material (45.9 mmol) was dissolved in n-butanol (200 ml) and treated with 1,2-diaminoethane (50 ml) at 100° C. After stirring at 100° C. until completion, the reaction mixture was concentrated in vacuo, coevaporated with toluene three times and dried under high vacuum. Final purification was achieved by silica gel chromatography. The typical yield for the product formation varied between 78 and 92%.

Standard Operating Procedure 31: Introduction of a Thiocresyl Ether at the Reducing End

A solution of the 1-O-glycosyl acetate (10.48 mmol) and p-thiocresol (12.58 mmol) in dry dichloromethane (30 ml) was stirred at 0° C. and subsequently activated by the addition of boron trifluoride diethylether complex (12.58 mmol) over 5 min. Stirring was continued (0° C.→20° C.) until completion and the reaction stopped by the addition of triethyl amine (14.0 mmol). The reaction mixture was diluted with dichloromethane and extracted with saturated NaHCO₃-solution, water and saturated brine solution, dried over MgSO₄ and evaporated in vacuo. Final purification was achieved by crystallization or silica gel chromatography. The typical yield for the product formation varied between 81 and 92%.

Standard Operating Procedure 32: Glycosylation with Thioglycosides a) NIS-Promoted Glycosylation

A mixture of glycosyl acceptor (1 mmol), thioglycoside (1 mmol) and 1.0 g. of freshly activated molecular sieves in 20 ml of a dry solvent (e.g. CH₃CN, CH₂Cl₂, Toluene, Ether) was stirred for 45 min at r.t and cooled down to the reaction temperature. Subsequently, N-Iodosuccinimide (1.7 mmol) was added and stirring continued for 20 min at the reaction temperature. After the addition of a Lewis acid as promotor (e.g. TfOH, 85-170 μmol), stirring was continued at the reaction temperature until completion. The reaction mixture was quenched with triethyl amine, filtered through a celite pad and extracted with a 10% aqueous KHCO₃/Na₂S₂O₃ solution, water and saturated brine solution, dried over MgSO₄ and evaporated. Final purification was achieved by silica gel column chromatography. The typical yields for the product formation varied between 65 and 85%.

b) DMTST Promoted Glycosylations:

A mixture of glycosyl acceptor (1 mmol), thioglycoside (1 mmol) and 1.0 g of freshly activated molecular sieves in 20 ml of a dry solvent (e.g. CH₃CN, CH₂Cl₂, Toluene, Ether) was stirred for 45 min at r.t and cooled down to the reaction temperature. Subsequently, DMTST (3-5 equiv.) was added stirring continued at the reaction temperature until completion. The reaction mixture was quenched with triethyl amine, filtered through a celite pad and extracted with aqueous NaHCO₃-solution, water and saturated brine solution, dried over Na₂SO₄, concentrated in vacuo and purified by silica gel column chromatography. The typical yields for the product formation varied between 50 and 85%.

Standard Operating Procedure 33:

Glycosylations with Trichloroacetimidates

A suspension of the trichloroacetimidate (1.54 mmol), glycosyl acceptor (11.3 mmol) and freshly activated molecular sieves (1.0 g) in an anhydrous solvent (e.g. CH₃CN, CH₂Cl₂, Toluene, Ether, 20 mL) was stirred at rt for 1 h and then cooled to reaction temperature. Subsequently, a catalytic amount of a promotor (e.g. TMSOTf, 0.01-0.1 equiv.) was added and stirring continued at reaction temperature until completion. The reaction was quenched with triethylamine) and filtered through a Celite pad. The combined organic layers were washed with aqueous NaHCO₃-solution and saturated brine solution, dried over Na₂SO₄, concentrated in vacuo and purified by silica gel column chromatography. The typical yields for the product formation varied between 50 and 85%.

Standard Operating Procedure 34:

Glycosylations Using 2,3-cyclocarbamoyl Protected pThiocresyl Glycosides as Glycosyl Donors

PhSCl (0.2 mmol, 2 equiv.) in dry dichloromethane (1 ml) was added dropwise to a mixture of AgOTf (0.2 mmol) in dry dichloromethane (2 ml) at −78° C. containing freshly activated molecular sieves 3 Å. After stirring for 15 mins at −78° C., a solution of the thioglycoside (0.1 mmol, 1 equiv.) and DTBMP (0.2 mmol, 2 equiv.) in dry dichloromethane (2 ml) was slowly added. After further stirring for 15 mins at −78° C., the glycosyl acceptor (0.2 mmol, 2 equiv.) in dry dichloromethane (1 ml) was slowly added and stirring continued until completion. The reaction was quenched with saturated aqueous NaHCO₃ solution (1 ml), warmed to rt and diluted with dichloromethane. The organic layer was dried over MgSO₄, filtered and evaporated. Final purification was achieved by silica gel chromatography. The typical yields for the product formation varied between 60 and 90%.

Standard Operating Procedure 35: Introduction of an Alloc-Group

A solution of starting material (2 mmol), dry pyridine (5 mmol) and dry THF (5 ml) was cooled to 0° C. Subsequently, Allylchloroformate (2.2 mmol) were added dropwise and stirring was continued until completion. The reaction mixture was diluted with dichloromethane and subsequently washed with 10% aqueous citric acid solution, saturated NaHCO₃ solution, water and saturated brine solution. The organic layer was dried over Na₂SO₄, filtered and evaporated. Final purification was achieved either by crystallization or by silica gel chromatography. The typical yields for the product formation varied between 80 and 95%.

Standard Operating Procedure 36: Cleavage of an Alloc-Group

A mixture of the Allyloxycarbonate (1.17 mmol), dimedone (1.33 mmol) and Pd(Ph₃P)₄ (0.30 mmol) was dissolved in dry THF (60 ml) and stirred under Ar atmosphere until completion of the reaction. The reaction mixture was concentrated in vacuo and purified by silica gel chromatography. The typical yields for the product formation varied between 78 and 97%.

Standard Operating Procedure 37: Lewis Acid Mediated Benzylation

To a stirred mixture of the starting material (1 mmol) and benzyl trichloroacetimidate in dry hexane/dichloromethane (10 ml, 2/1) was added Lewis acid (0.01-0.05 equiv., e.g. TMSOTf, TfOH) and stirring was continued at rt until completion. The reaction was quenched with triethyl amine and concentrated. Final purification was achieved by silica gel chromatography. The typical yields for the product formation varied between 50 and 92%.

Standard Operating Procedure 38: Benzylation Under Mild Basic Conditions

The starting material (3.49 mmol) was dissolved in dry DMSO (20 ml) and cooled to 0° C. To the stirred solution were added successively benzyl bromide (3.5 equiv./OH-group), barium oxide (1.5 equiv/OH-group), catalytic amounts of TBAI (0.05 eqiv./OH-group) and potassium hydroxide (3.5 equiv./OH-group). Stirring was continued from 0° C. to rt until completion. The reaction was quenched with methanol, and further stirred for 30 min. After dilution with ether, the organic layer was washed with water and brine solution, dried over MgSO₄ and concentrated in vacuo. Final purification was achieved by silica gel chromatography.

Standard Operating Procedure 39: Ester Cleavage Under Aqueous Conditions

The starting material (0.3 mmol ester groups) was dissolved in 11.8 ml of a mixture of water and THF (3:7), cooled to 0° C. and reacted with 1M aqueous NaOH-solution (5.0 ml). Stirring was continued until completion and the reaction mixture titurated with 10% aqueous HCl-solution to a pH of 9.5. After evaporation of the THF, the mixture was freeze dried and the remaining residue purified by silica gel chromatography to yield the product. The typical yields for the product formation varied between 85 and 95%.

EXAMPLE 1 Synthesis of Building Blocks A-1 and A-2 from N-Acetyl Glucosamine

Example 1: Synthesis of building blocks A-1 and A-2 from N-Acetyl glucosamine, yields are reported for R=H, conditions; a) Amberlyte IR 120 (H⁺), MeOH, 60° C., (70%); b) 1M NaOH, 120° C.; c) 1. SOP 10; 2. Ac₂O, pyridine; 3. NaOMe, MeOH (70%, 4 steps); d) SOP 1 (80% for R=H) or SOP 2 for R=OMe; e) SOP 11; f) SOP 12, (85%, 2 steps); g) SOP 7, (92%); h) SOP 4, (91%); i) SOP 17, (85%); j) SOP 5.

Preparation of A-1-i:

N-Acetyl-2-deoxy-α/β-D-glucopyranoside (8.5 g, 38.4 mmol) was suspended in 100 ml dry methanol. Subsequently, 12.0 g Amberlite IR 120 iron exchange resin (H⁺-form) was added and the reaction mixture refluxed for 70 hrs at 65° C. After cooling to 25° C., the iron exchange resin was removed by filtration and several times extracted with methanol. The combined methanol layers were neutralized with triethyl amine and concentrated in vacuo. The crude residue was purified by crystallization to furnish the title compound in 70% yield (α/β-mixture).

Preparation of A-1-iii:

Methyl glycoside A-1-i (20.6 mmol) was suspended in 100 ml aqueous NaOH solution (1 M) and stirred under reflux at 120° C. until completion. After cooling and neutralization with 10% aqueous HCl, the mixture was concentrated in vacuo and crude A-1-ii suspended in 200 ml methanol and reacted with N-[(1,3-dimethyl-2,4,6 (1H,3H,5H)-trioxopyrimidin-5-ylidene)methyl]-N′,N″-dime-thylamine (23.6 mmol) at 50° C. (pH˜9.0) until completion. After evaporation and drying, crude A-1-iii was reacted with 150 mL acetylation mixture (pyridine/Ac₂O, 2/1, v/v) until completion, concentrated in vacuo, coevaporated with toluene and dried. The residue was suspended in ethyl acetate and extracted with water, 10% aqueous HCl, saturated, aqueous NaHCO₃ solution and H₂O, dried over Na₂SO₄ and concentrated. The crude residue was dissolved in dry methanol and reacted with a catalytic amount of NaOMe. After completion, the reaction was neutralized with Amberlite IR 120 and filtered. The organic layer was evaporated and dried to furnish the title compound A-1-iii in 70% yield (over 4 steps).

Preparation of A-1-iva:

Methyl-2-deoxy-2-N-[1-(1,3-dimethyl-2,4,6(1H, 3H, 5H)-trioxopyrimidin-5-ylidene) methyl]-α-D-glucopyranoside A-1-iii (16.0 g, 44.5 mmol) in acetonitrile (200 ml) was reacted with benzaldehyde dimethyl acetal (14.0 ml, 89.0 mmol) and a catalytic amount of p-toluenesulphonic acid monohydrate. After 2 hours at 55° C., the mixture was neutralized and evaporated. The remaining residue was extracted, washed and evaporated. Yield: 18.3 g (92%), R_(f)=0.20 (1,2-dichloroethane/ethylacetate=7/3).

Preparation of A-1-va:

Methyl-4,6-O-benzylidene-2-deoxy-2-N-[1-(1,3-dimethyl-2,4,6(1H, 3H, 5H)-trioxopyrimidin-5-ylidene)methyl]-α-D-glucopyranoside A-1-iva (18.30 g, 40.94 mmol) in DMF (50 ml) was reacted with ethylenediamine (20 ml) at room temperature. After stirring for 35 minutes, the mixture was concentrated. Yield: 10.90 g (94.7%), R_(f)=0.18 (chloroform/methanol=9/1).

Preparation of A-1-via:

To a solution of methyl-2-amino-4,6-O-benzylidene-2-deoxy-α-D-glucopyranoside (7.5 g, 26.0 mmol) and 4-N,N′-(dimethylamino)pyridine (14.5 g) in acetonitrile (100 mL) was added TfN₃-solution (85 ml) at room temperature. The reaction mixture was concentrated and the residue was purified by filtration through a short silica gel pad. Yield: 7.00 g (85.3%), R_(f)=0.18 (chloroform/methanol=9/1).

Preparation of A-1-viia:

Methyl 2-azido-2-deoxy-4,6-benzylidene-α-D-glucopyranoside A-1-via (10.87 g, 35.41 mmol) in N,N′-dimethylformamide (50 mL) was reacted with NaH (95%, 0.92 g, 42.49 mmol) and benzyl bromide (5.47 ml, 45.9 mmol). After completion, the excess of NaH was quenched, followed by concentration. The residue was extracted, washed and concentrated. Yield: 12.93 g (92.0%), R_(f)=0.37 (petroleum spirit/ethyl acetate=3/1).

Preparation of A-1:

Methyl-2-azido-3-O-benzyl-2-deoxy-α-D-glucopyranoside (9.87 g, 32.04 mmol) in dichloromethane (50 mL) and pyridine (10 mL) was treated with benzoyl chloride (3.72 mL, 32.04 mmol) at −45° C. for 2 hours. The reaction was concentrated and the residue extracted, washed and evaporated. Yield: 10.78 g (81.7%), R_(f)=0.31 (petroleum spirit/ethylacetate=1/1).

Compound A-1:

¹H-NMR (400 MHz, CDCl₃): δ=8.03 (d, 2H, Aryl), 7.57 (m, 1H, Aryl), 7.45-7.29 (m, 7H, Aryl), 4.93 (d, 1H, J_(gem)=10.8 Hz, OCH₂), 4.82 (d, 1H, J_(gem)=10.8 Hz, OCH₂), 4.81 (d, 1H, J_(1,2)=3.6 Hz, H-1α), 4.73 (dd, 1H, J_(5,6a)=4.4 Hz, J_(gem)=12.0 Hz, H-6a), 4.47 (dd, 1H, J_(5,6b)=2.0 Hz, H-6b), 3.85 (dd, 1H, J_(3,4)=8.8 Hz, H-3), 3.57 (ddd, 1H, J_(4,5)=10.0 Hz, H-4), 3.45 (s, 3H, OMe), 3.37 (dd, 1H, J_(2,3)=10.0 Hz, H-2), 2.80 (bs, 1H, 4-OH).

EXAMPLE 2 Synthesis of Building Blocks A-3 and A-4

Example 2: Synthesis of building block A-3 and A-4, conditions: a) SOP 7, (72% for R=H); b) SOP 4, (82%); c) SOP 17, (84%); d) SOP 5.

Compound A-3:

¹H-NMR (400 MHz, CDCl₃): δ=10.16 (dd, 1H, J_(NH,2)=9.4 Hz, J_(NH, ═C—H)=14.0 Hz, NH), 8.11 (d, 1H, ═C—H), 7.68-7.22 (3m, 8H, Aryl), 4.84 (d, 1H, J_(1,2)=3.5 Hz, H-1α), 4.83 (dd, 1H, J_(6a, 6b)=12.3 Hz, J_(5,6a)=3.5 Hz, H-6a), 4.73 (d, 1H, J_(gem)=11.7 Hz, OCH₂), 4.46 (dd, 1H, J_(5,6b)=2.1 Hz, H-6b), 3.91 (m, 1H, H-5), 3.72 (dd, 1H, J_(3,4)≈J_(2,3)=8.8 Hz, H-3), 3.57 (ddd, 1H, J_(4,5)=9.5 Hz, H-4), 3.48 (s, 3H, OMe), 3.38 (ddd, 1H, J_(2,3)=10.5 Hz, H-2), 3.32 (s, 3H, NMe), 3.31 (s, 3H, NMe), 3.05 (bs, 1H, 4-OH).

EXAMPLE 3 Synthesis of L-ido Configured Glycosyl Donor B-1

Example 3: Synthesis of Building Block B-1, conditions: a) SOP 7, (95%); b) 60% aqueous Acetic acid, 60° C. (90%); c) Methanesulfonyl chloride, Pyridine, 0° C.-RT (87%); d) Cesium Acetate, Ac₂O, 120° C. (95%); e) SOP 22, (92%); f) 1.90% TFA, 0° C.; 2. Ac₂O, Pyridine; 3. SOP 31, (73%, 3 steps); g) SOP 22, (98%); h) SOP 3, (92%); i) SOP 18, (98%); j) 80% acetic acid, 100° C. (98%); k) SOP 26, (89%); I) SOP 23, (98%).

Preparation of B-1-iii:

B-1-ii (15.60 mmol) was dissolved in 60% aqueous acetic acid (50 ml) and stirred at 60° C. until completion. After neutralization with solid NaHCO₃, the mixture was evaporated and co evaporated with toluene. Crude B-1-iii was dissolved in CHCl₃/H₂O, the organic layer separated, dried over Na₂SO₄ and evaporated. The remaining residue was purified by a short silica gel chromatography to yield B-1-iii in 90% (4.43 g).

Preparation of B-1-iv:

17.72 mmol of B-1-iii was dissolved in 25 ml dry pyridine, to which mesyl chloride (methylsulfonyl chloride, 42.5 mmol) was added dropwise at 0° C. The mixture was stirred at 4° C. until completion and was subsequently poured into warm water (50° C., 90 ml), cooled and the precipitate isolated by filtration. B-1-iv was obtained after drying in 87% yield (7.23 g).

Preparation of B-1-v:

B-1-iv (6.43 mmol) and cesium acetate (64.3 mmol) were suspended in 25 ml acetic anhydride and refluxed at 125° C. until completion. The reaction mixture was concentrated in vacuo, co evaporated with toluene and the residue extracted from ethyl acetate/H₂O (1/1). The organic layer was collected and washed with saturated aqueous NaHCO₃ solution and saturated brine solution, dried over Na₂SO₄ and evaporated. Purification was achieved by silica gel chromatography. Yield: 2.68 g (95%).

Preparation of B-1-vii:

B-1-vi (5.61 mmol) was dissolved in aqueous TFA (90%, 15 ml) and further stirred at 0° C. until completion. The reaction mixture was neutralized with aqueous NaOH solution at 0° C., concentrated in vacuo and dried. The residue was suspended in 90 ml acetylation mixture (pyridine/acetic anhydride=2/1) and 50 ml dichloromethane at 0° C. and further stirred until completion. After concentration in vacuo and co evaporation with toluene, the residue was dissolved in ethyl acetate/H₂O (1/1), the organic layer collected and washed with 10% aqueous citric acid solution, saturated aqueous NaHCO₃ solution and brine solution, dried over Na₂SO₄ and evaporated. The crude residue and p-thiocresol (6.0 mmol) were dissolved in 40 ml anhydrous dichloromethane and cooled to 0° C., reacted with BF₃xOEt₂ (8.41 mmol) and further stirred at rt until completion. The reaction was stopped with saturated NaHCO₃ solution and the organic layer was washed with water, dried over Na₂SO₄ and evaporated. Final purification was achieved by silica gel chromatography to yield B-1-vii in 73% over 3 steps.

Preparation of B-1-xi:

B-1-x (8.0 mmol) was dissolved in 80% aqueous AcOH and heated at 100° C. until completion. The mixture was cooled to rt, neutralized with solid NaHCO₃ and dissolved in ethyl acetate/water (1/1). After removal of the aqueous layer, the organic layer was dried over MgSO₄ and evaporated to dryness furnishing B-1-xi in 98% yield.

Compound B-1:

¹H-NMR (400 MHz, CDCl₃): δ=8.07 (d, 2H, Aryl), 7.57-7.30 (m, 10H, Aryl), 7.10 (d, 2H, Aryl), 6.88-6.81 (m, 4H, Mp), 5.55 (d, 1H, J_(1,2)<1.5 Hz,H-1β), 5.45 (m, 1H, H-2), 5.26 (ddd, 1H, H-5), 5.13 (m, 1H, H-4), 4.91 (d, 1H, J_(gem)=12.1 Hz, OCH₂), 4.78 (d, 1H, J_(gem)=12.1 Hz, OCH₂), 4.16 (dd, 1H, J_(gem)=9.6 Hz, J_(5,6a)=7.6 Hz, H-6a), 4.08 (dd, 1H, J_(5,6b)=5.2 Hz, H-6b), 3.93 (m, 1H, H-3), 3.77 (s, 3H, OCH₃), 2.58-2.36 (m, 4H, (CH)₂ Lev), 2.32 (s, 3H, SCH₃), 2.05 (s, 3H, CH₃C═O).

EXAMPLE 4 Synthesis of L-ido configured Glycosyl Donor B-2.

Example 4: Synthesis of L-ido configured glycosyl donor B-2; a) SOP 2; b) SOP 18; c) SOP 5; d) SOP 23.

EXAMPLE 5 Synthesis of Building Block C-1, C-1a and C-1b

Example 5 Synthesis of Building Blocks C-1, C-1a and C-1b, conditions: a) SOP 19; b) SOP 13, (78%, 2 steps); c) SOP 8, (91%); d) SOP 22; e) SOP 2, (85%, 2 steps for C-1-vi); f SOP 18; g) SOP 5, (75%, 2 steps for C-1).

Preparation of C-1-iia:

To methyl 2-azido-2-deoxy-1-thio-β-D-glucopyranoside. (10 g, 42.55 mmol) in pyridine (50 ml) at 0° C. was added acetic anhydride (20 g) and the reaction stirred for 1 hour. The reaction mixture was evaporated to dryness and the residue extracted to give the title triacetate (15.23 g, quantitative,), R_(f)=0.7 (CHCl₃/Petroleum ethers, 1:1).

Preparation of C-1-iii:

To a solution of methyl 3,4,6-tri-O-acetyl-2-azido-2-deoxy-1-thio-β-D-glucopyranoside in wet acetone (200 ml) was added NBS (3 equiv.). The resulting mixture was allowed to stir for 2 h. The mixture was then quenched, concentrated and the residue purified by silica gel chromatography to give the title hemiacetal as an oil (10.1 g, 78%), R_(f)=0.5 (EtOAc/Petroleum ether, 1:1).

Preparation of C-1-vi:

A mixture of 2-azido-2-deoxy-β-D-glucopyranosyl tert-butyldiphenylsilane (5.5 g, 12.42 mmol), 4-methoxybenzaldehyde dimethylacetal (4.4 g, 24 mmol), and 4-toluenesulphonic acid (100 mg,) in acetonitrile/DMF (200 ml, 5:3) were heated at 60° C. for 1 hour. The reaction mixture was then neutralized and evaporated to give the crude compound as an oil. The residue was purified by silica chromatography to give the product (6.7 g, 96%, 85% from C-1-iv); f=0.8 (dichloromethane/Petroleum ethers; 10:2).

Preparation of C-1-vii:

A mixture of DMAP (1.63, 13.6 mmol) and benzoyl chloride (1.7 g, 12.1 mmol) and 2-azido-2-deoxy-4,6-O-(4-methoxybenzylidene)-β-D-glucopyranosyl tert-butyldiphenylsilane (6.7 g, 11.9 mmol) in 1,2-dichloroethane (100 ml) was stirred at 60° C. for 1 h. The reaction mixture was quenched, extracted, washed and concentrated to give a crude residue. The residue was passed through a plug of silica to give the product (5.5 g, 69%); R_(f)=0.7 (dichloromethane/Petroleum ethers; 4:1).

Preparation of C-1:

To a mixture of 2-azido-2-deoxy-3-O-benzoyl-4,6-O-(4-methoxybenzylidene)-β-D-glucopyranosyl tert-butyldiphenylsilane (10 g, 15 mmol), sodiumcyanoborohydride (5 g, 80 mmol) and molecular sieves in DMF (200 ml) at 0° C. was added trifluoroacetic acid (28 g, 247 mmol) at 0° C. and then left to run overnight at rt. The reaction mixture was quenched, filtered and concentrated and the residue purified by column chromatography to give the title compound (7.0 g, 70%), R_(f)=0.4 (ethylacetate/petroleum ethers, 3:7).

Compound C-1:

¹H-NMR (400 MHz, CDCl₃):δ=8.08 (d, 2H, Aryl), 7.72 (m, 4H, Aryl), 7.59 (m, 1H, Aryl), 7.47 (m, 3H, Aryl), 7.42 (m, 2H, Aryl), 7.34 (m, 3H, Aryl), 7.13 (d, 2H, Mpm) 6.83 (d, 2H, Mpm), 4.96 (dd, 1H, J_(2,3)≈J_(3,4)=9.7 Hz, H-3), 4.53 (d, 1H, J_(1,2)=7.6 Hz, H-1β), 4.37 (2d, 2H, OCH₂), 3.83 (ddd, 1H, H-4), 3.79 (s, 3 H, OCH₃), 3.65 (dd, 1H, H-2), 3.53 (dd, 1H, J_(gem)=10.8 Hz, J_(5,6a)=4.1 Hz, H-6a), 3.46 (dd, 1H, J_(5,6b)=4.1 Hz, H-6b), 3.12 (m, 1H, H-5), 3.02 (d, 1H, J_(4,OH) =3.5 Hz, 4-OH), 1.12 (s, 9H, C(CH₃)₃).

EXAMPLE 6 Synthesis of Building Block C-2

Example 6 Synthesis of Building Block C-2, conditions: a) SOP 1, (90% for R=SMe); b) SOP 18, (87% for R=SMe); c) SOP 4, p-TosOH, MeOH, CH₃CN (86% for R=SMe); d) SOP 17, (92% for R=SMe); e) SOP 13, (94%); f) SOP 8, (82%).

Compound C-2:

¹H-NMR (400 MHz, CDCl₃): δ=8.09 (d, 2H, Aryl), 7.97 (d, 2H, Aryl), 7.72 (m, 4H, Aryl), 7.60 (m, 1H, Aryl), 7.50-7.27 (m, 11H, Aryl), 4.98 (dd, 1H, J_(2,3)=J_(3,4)=9.7 Hz, H-3), 4.58 (d, 1H, J_(1,2)=7.8 Hz, H-1β), 4.51 (dd, 1H, J_(gem)=11.3 Hz, J_(5,6a)=4.7 Hz, H-6a), 4.36 (dd, 1H, J_(5,6b)=2.2 Hz, H-6b), 3.72-3.68 (m, 2 H, H-2, H-4), 3.31 (m, 1H, H-5), 3.23 (d, 1H, J_(4,OH)=4.5 Hz, 4-OH), 1.13 (s, 9 H, C(CH₃)₃).

EXAMPLE 7 Synthesis of Several Carbamoylated Building Blocks C-3a to C-3d and C-4a to C-4d, Containing a 6-O Benzoyl or 6-O-p-methoxybenzyl Protection

Example 7 Synthesis of several carbamoylated building blocks C-3a to C-3d and C-4a to C-4d, containing a 6-O-benzoyl or 6-O-p-methoxybenzyl protection, conditions: a) R=MeO: SOP 2; R=H: SOP 1, (82%, R¹=SCres, R=H); b) SOP 30, (87%, R¹=SCres, R=H); c) SOP 29, (95%, R¹=Scres, R=H); d) SOP 4, (72%, R¹=SCres); e) SOP 17, (85%); f) SOP 5.

Compound C-3a:

¹H-NMR (400 MHz, CDCl₃): δ=8.06 (d, 2H, Aryl), 7.62 (m, 1H, Aryl), 7.48 (t, 2H, Aryl), 7.38 (d, 2H, Aryl), 6.97 (d, 2H, Aryl), 5.06 (bs, 1H, NH), 4.79 (dd, 1H, J_(gem)=12.0 Hz, J_(5,6a)=3.6 Hz, H-6a), 4.70 (d, 1H, J_(1,2)=9.2 Hz, H-1β), 4.63 (dd, 1H, J_(5,6b)=2.0 Hz, H-6b), 4.18 (dd, 1H, J_(2,3)≈J_(3,4)=10.4 Hz, H-3), 3.89 (dd, 1H, J_(4,5)=9.2 Hz, H-4), 3.72 (m, 1H, H-5), 3.23 (ddd, 1H, H-2), 3.12 (bs, 1H, 4-OH), 2.29 (s, 3H, SCH₃).

EXAMPLE 8 Synthesis of Several 6-OMp and Cyclic 2,3-Carbamoyl Protected Building Blocks C-5a to C-5c

Example 8 Synthesis of several 6-OMp and cyclic 2,3-carbamoyl protected building blocks C-5a to C-5c, conditions: a) SOP 18, (92% for R¹=OTBDPS); b) SOP 4, (82%); c) SOP 26, (75% for R¹-OTBDPS); d) SOP 30, (87%); e) SOP 29, (95%).

Compound C-5c:

¹H-NMR (400 MHz, CDCl₃): δ=7.69 (m, 2H, Aryl), 7.63 (m, 2H, Aryl), 7.46-7.31 (m, 6H, Aryl), 6.82 (bs, 4H, Mp), 5.04 (bs, 1H, NH), 4.78 (d, 1H, J_(1,2)=7.6 Hz, H-1β), 4.15-4.10 (m, 3H, H's not assigned), 3.97 (dd, 1H, J=11.6 Hz, J=9.6 Hz, H not assigned), 3.78 (s, 3H, OMe), 3.56 (m, 1H, H not assigned), 3.48 (m, 1H, H not assigned), 2.80 (bs, 1H, 4-OH), 1.08 (s, 9H, C—(CH₃)₃).

EXAMPLE 9 Synthesis of Building Blocks C-6-a and C-6-b and C-7

Example 9: Synthesis of building blocks C-6-a, C-6-b and C-7, conditions; a) SOP 12, (83%); b) SOP 7; c) SOP 5, (75%, 2 steps); d) SOP 14 (82%); e) SOP 8 (91%).

Compound C-6-a:

¹H-NMR (400 MHz, CDCl₃): δ=7.43 (d, 2H, Aryl), 7.25 (m, 4H, Aryl), 7.08 (d, 2H, Aryl), 6.88 (m, 4H, Aryl), 4.81 (d, 1H, J_(gem)=10.8 Hz, OCH₂), 4.74 (d, 1 H, J_(gem)=10.8 Hz, OCH₂), 4.53 (d, 1H, J_(gem)=11.1 Hz, OCH₂), 4.48 (d, 1H, J_(gem)=10.8 Hz, OCH₂), 4.35 (d, 1H, J_(1,2)=10.0 Hz, H-1β), 3.82 (s, 3H, OCH₃), 3.79 (s, 3H, OCH₃), 3.76 (dd, 1H, J_(gem)=10.5 Hz, J_(5,6a)=5.4 Hz, H-6a), 3.70 (dd, 1H, J_(5,6b)=5.4 Hz, H-6b), 3.56 (ddd, 1H, H-4), 3.42 (m, 1H, H-5), 3.34 (dd, 1H, J_(3,4)=8.8 Hz, H-3), 3.24 (dd, 1H, J_(2,3)=9.4 Hz, H-2), 2.72 (d, 1H, J_(4,OH)=3.5 Hz, 4-OH), 2.38 (s, 3H, SCH₃).

Compound C-7:

¹H-NMR (400 MHz, CDCl₃): 7.63 (d, 4H, Aryl), 7.35-7.21 (m, 8H, Aryl), 7.08 (m, 2H, Aryl), 6.83-6.78 (m, 4H, Aryl), 4.72 (d, 1H, J_(gem)=11.0 Hz, OCH₂), 4.59 (d, 1H, J_(gem)=11.0 Hz, OCH₂), 4.29 (d, 1H, J_(1,2)=7.8 Hz, H-1β), 4.27 (d, 1H, J_(gem)=11.7 Hz, OCH₂), 4.21 (d, 1H, J_(gem)=11.7 Hz, OCH₂), 3.72 (s, 3H, OCH₃), 3.71 (s, 3H, OCH₃), 3.51 (ddd, 1H, J_(3,4)≈J_(4,5)=8.6 Hz, H-4), 3.40-3.32 (m, 3H, H-6a, H-6b, H-2), 3.05 (dd, 1H, J_(2,3)=9.8 Hz, H-3), 2.90 (m, 1H, H-5), 2.51 (d, 1H, J_(4,OH)=2.2 Hz, 4-OH), 1.12 (s, 9H, C(CH₃)₃).

EXAMPLE 10 Synthesis of Building Block C-8a to C-8c

Example 10 Synthesis of building blocks C-8a to C-8c, conditions: a) SOP 7, AlIBr, DMF (65%, R=OTBDPS); b) SOP 4, (86%, R=OTBDPS); c) SOP 26, (70%, R=OTBDPS); d) SOP-30; e) SOP 12, (70%, 2 steps for R=OTBDPS).

Compound C-8c:

¹H-NMR (400 MHz, CDCl₃): δ=7.72 (m, 4H, Aryl), 7.43-7.16 (m, 6H, Aryl), 6.76 (m, 4H, Mp), 5.96 (m, 1H, ═CH Allyl), 5.31 (m, 1H, ═CH Allyl), 5.22 (m, 1H, ═CH Allyl), 4.42 (d, 1H, J_(1,2)=7.6 Hz, H-1), 4.39 (m, 1H, OCH₂ Allyl), 4.23 (m, 1H, OCH₂ Allyl), 3.97 (dd, 1H, J_(gem)=10.0 Hz, J_(5,6a)=3.6 Hz, H-6a), 3.92 (dd, 1H, J_(5,6b)=5.2 Hz, H-6b), 3.77 (s, 3H, OCH₃), 3.66 (ddd, 1H, J_(4,5) ≈J_(3,4)=9.4 Hz, H-4), 3.42 (dd, 1H, J=9.8 Hz and J=7.8 Hz, H not assigned), 3.22 (m, 1H, H-5), 3.09 (dd, 1H, J=8.4 Hz and J=9.6 Hz, H not assigned), 2.48 (d, 1H, J_(4,OH)=2.8 Hz, 4-OH), 1.12 (s, 9H, C(CH₃)₃).

EXAMPLE 11 Synthesis of Building Block D-1:

Example 11: Synthesis of building block D-1; a) SOP 1, (95%); b) SOP 7, (85%); c) SOP 13, (92%); d) SOP 8; e) SOP 4, (70%, 2 steps); f 1. SOP 15; 2. SOP 16, (75%, 2 steps).

Compound D-1:

¹H-NMR (400 MHz, CDCl₃): δ=7.72 (m, 4H, Aryl), 7.41 (m, 2H, Aryl), 7.32-7.25 (m, 14H, Aryl), 5.04 (d, 1H, J_(gem)=11.0 Hz, OCH₂), 4.81 (m, 3H, OCH₂), 4.63 (d, 1H, J_(1,2)=7.4 Hz, H-1β), 3.88 (ddd, 1H, J_(3,4)≈J_(4,5)=9.2 Hz, H-4), 3.70 (s, 3H, OCH₃), 3.53 (dd, 1H, J=7.5 Hz, J=9.0 Hz, H not assigned), 3.47 (d, 1H, J_(4,5)=9.8 Hz, H-5), 3.42 (dd, 1H, J=8.9 Hz and J=8.9 Hz, H not assigned), 2.87 (d, 1H, J_(4,OH)=2.4 Hz, 4-OH), 1.11 (s, 9H, C(CH₃)₃).

EXAMPLE 12 Synthesis of Building Block D-2, a 2-O-Allyloxycarbonyl Protected Thioethyl Glycoside

Example 12: Synthesis of Building Block D-2, conditions: a) 1. SOP 22; 2. SOP 1; b) SOP 7; c) 1. SOP 4; 2. TritCl, Pyridine, (ClCH₂)₂; 3. SOP 23; d) 1. CrO₃, H₂SO₄, Acetone, 0° C., 2. SOP 16; e) 1. Dimethyl dioxirane, Acetone; 2. EtSH, TFAA, CH₂Cl₂, 3. SOP 35.

EXAMPLE 13 Synthesis of Building Block D-3

Example 13: Synthesis of Building Block D-3, conditions: a) 1. SOP 4, Amberlite IR 120, H₂O, 80° C.; 2. SOP 18, (85%, 2 steps) b) 1. SOP 21; 2. SOP 8, (70%, 2 steps); c) SOP 22, (96%); d) 1. SOP 15; 2. SOP 16, (80%, 2 steps); e) SOP 23, (92%); f) SOP 9, (95%); g) SOP 25a, (91%).

Preparation of D-3-i. Step 1:

The starting material (57 mmol) and Amberlite IR 120 iron exchange resin (H⁺-form, 20 g) were suspended in water (180 ml) and stirred at 80° C. until completion. The iron exchange resin Us removed by filtration and extracted with water The combined aqueous layers were neutralized with triethyl amine and freeze dried.

Compound D-3-V:

¹H-NMR (400 MHz, CDCl₃): δ=7.95 (m, 2H, Aryl), 7.68 (m, 2H, Aryl), 7.58-7.12 (m, 16H, Aryl), 5.47 (dd, J_(1,2)=7.6 Hz, J_(2,3)=9.6 Hz, H-2), 5.31 (dd, J_(3,4)=9.6 Hz, H-4), 4.64 (d, 1H, 7.6 Hz, H-1β), 4.60 (d, 1H, J_(gem)=12.0 Hz, OCH₂), 4.55 (d, 1H, J_(gem)=12.0 Hz, OCH₂), 3.74 (dd, 1H, H-3), 3.70 (s, 3H, OCH₃), 3.63 (d, 1H, J_(4,5)=9.6 Hz, H-5), 2.68-2.16 (m, 4H, (CH₂)₂-Lev), 2.15 (s, 3H, CH₃), 0.96 (s, 9H, C(CH₃)₃).

EXAMPLE 14 Syntheses of a Range of Block D Donor Sugars D-4 to D-7 from a Common Intermediate, a 4-O-levulinoyl Glucal

Example 14: Syntheses of D-4 to D-7 as donor sugars, conditions: a) 1. Dimethyl dioxirane, Acetone; 2. TBAF, THF; 3. SOP 35; b) 1. Dimethyl dioxirane, Acetone; 2. 4-penten-1-ol, ZnCl₂, CH₂Cl₂; 3. SOP 35; c) 1. Dimethyl dioxirane, Acetone; 2. ArSH, TFAA, CH₂Cl₂, (Ar=Ph, p-Tol); 3. SOP 35 or (ClAc)₂O, Pyridine, CH₂Cl₂ (for D-6b); d) MCPBA, CH₂Cl₂ (for D-6b as substrate).

EXAMPLE 15 Synthesis of Building Block E-1 to E-4

Example 15 Synthesis of Building Block E-1 to E-4, conditions: a) SOP 8; b) SOP 7; c) SOP 9, (84% over 3 steps, R=SMe); d) SOP 18, (86%, R=SMe); e) 1. SOP 13, (75%, for E-1-iv-a as starting material); 2. SOP 25b, (88%); f) 1. TosCl, Pyridine; 2. p-MeO—C₆H₄—ONa, NMP, 60° C.; g) SOP 7, (78%, R=SMe); h) 1. SOP 14; 2. SOP 25b, (79%, 2 steps, R=SMe).

Preparation of E-1-1-a:

A mixture of methyl 2-azido-2-deoxy-thio-β-D-glucopyranoside (10 g, 42.5 mmol) and imidazole (4.9 g, 72.25 mmol) in 20 mL DMF was treated with tert-butyldiphenylchlorosilane (11.6 mL, 44.63 mmol) for 2 h. The reaction mixture was concentrated, extracted, washed and dried. yield: 23 g (crude light yellow syrup), R_(f)=0.74 (CHCl₃/methanol=9/1).

Preparation of E-1-ii-a:

The silyl ether from the previous step in 50 mL DMF, was treated with 2.68 g of 95% NaH (106.25 mmol) and 12.64 mL (106.25 mmol) of benzyl bromide at 0° C. After 1 h the excess NaH was quenched and the reaction concentrated, extracted, washed and concentrated to afford a yellow syrup yield: 28.5 g (crude yellow syrup), R_(f)=0.80 (hexane/ethyl acetate=7/3).

Preparation of E-1-iii-a:

The crude yellow syrup from the above reaction was treated with 36.5 mL AcOH and 106.3 mL (106.25 mmol) of 1M solution of TBAF in THF overnight. The reaction was concentrated and purified by chromatography to afford the title compound. 14.9 g (84%, 3 steps) R_(f)=0.36 (petroleum spirit/ethyl acetate=7/3).

Preparation of E-1-iv-a:

Methyl 2-azido-2,3 di-O-benzyl-2-deoxy-thio-β-D-glucopyranoside (14.5 g, 34.9 mmol) in dichloromethane (200 ml) and anhydrous pyridine (8.6 ml, 106.2 mmol) was treated with benzoylchloride (4.93 ml, 42.5 mmol) at 0° C. for 1 hour. The reaction mixture was quenched, extracted, washed and evaporated. The residue was purified by silica gel column chromatography to afford the title compound as a white solid.

Yield: 14.9 g (86%), R_(f)=0.82 (Petroleum spirit/Ethyl acetate=7/3).

Preparation of E-1:

Methyl 2-azido-6-O-benzoyl-2,3di-O-benzyl-2-deoxy-thio-?-D-glucopyranoside (8.68 g, 16.7 mmol) in acetone (50 ml) was treated with N-bromosuccinimide (8.92 g, 50.12 mmol) at 0° C. for 1 hour. The reaction mixture was then quenched, extracted, washed and evaporated, furnishing a yellow syrup which was purified by chromatography. Yield: 6.13 g (75%), R_(f)=0.57 (Petroleum spirit/Ethyl acetate=7/3). A cooled mixture of 2-azido-6-O-benzoyl-2,3 di-O-benzyl-2-deoxy-α/β-D-glucopyranose (5 g, 10.2 mmol), K₂CO₃ (7.0 g, 51 mmol) and trichloroacetonitrile (5.1 ml, 51 mmol) in 30 ml of dichloromethane was stirred for 2 h. The mixture was then filtered through celite and the filtrate was concentrated and purified on a short column of silica gel to obtain the title compound as an amorphous white solid. Yield 5.69 g (88%), R_(f)=0.85 (Petroleum spirit/Ethyl acetate=7/3).

Compound E-1:

¹H-NMR (400 MHz, CDCl₃): δ=8.73 (s, 1H, C═NH), 8.00 (m, 2H, Aryl), 7.56 (m, 1H, Aryl), 7.43-7.25 (m, 12H, Aryl), 5.66 (d, 1H, J=8.4 Hz, H-1β), 4.95 (d, 1H, J_(gem)=10.8 Hz, OCH₂), 4.87 (d, 2H, J=10.8 Hz, OCH₂), 4.62 (d, 2H, J_(gem)=10.8 Hz, OCH₂), 4.58 (dd, 1H, J_(gem)=12.4 Hz, J_(5,6a)=2.0 Hz, H-6a), 4.46 (dd, 1H, J_(5,6b)=3.6 Hz, H-6b), 3.77-3.72 (m, 3H, H-5, 2H not assigned), 3.62 (dd, 1H, J=8.3 Hz, J=9.7 Hz, H not assigned).

Compound E-2:

¹H-NMR (400 MHz, CDCl₃): δ=8.70 (s, 1H, C═NH), 7.38-7.22 (m, 10H, Aryl), 7.13 (m, 2H, Aryl), 6.83 (d, 2H, Mpm), 6.44 (d, 1H, J_(1,2)=3.5 Hz, H-1α), 4.93 (d, 1H, J_(gem)=10.5 Hz, OCH₂), 4.89 (d, 1H, J_(gem)=10.5 Hz, OCH₂), 4.78 (d, 1H, J_(gem)=10.5 Hz, OCH₂), 4.57 (d, 1H, J_(gem)=11.7 Hz, OCH₂), 4.51 (d, 1H, J_(gem)=11.7 Hz, OCH₂), 4.39 (d, 1H, J_(gem)=11.7 Hz, OCH₂), 4.02 (dd, 1H, J_(3,4) ≈J _(2,3)=9.5 Hz, H-3), 3.98 (m, 1H, H-5), 3.86 (dd, 1H, J_(4,5)=9.6 Hz, H-4), 3.76 (dd, 1H, H-2), 3.75 (s, 3H, OCH₃), 3.69 (dd, 1H, J_(5,6a)=3.5 Hz, J_(gem)=10.5 Hz, H-6a), 3.63 (dd, 1H, J_(5,6)=1.8 Hz, H-6b).

EXAMPLE 16 Synthesis of Building Blocks E-5 to E-8

Example 16 Syntheses of Building Blocks E-5 to E-8, conditions: a) SOP 6, (85%, R=SMe); b) SOP 30, (86%, R=SMe); c) SOP 10, (88%, R=SMe); d) SOP 18, (92%, R=SMe); e) 1. SOP 13; 2. SOP 25b, (85%, 2 steps, R=SMe); f) SOP 7; g) 1. SOP 14; 2. SOP 25b; h) 1. TsCl, DMF; 2. p-MeO—C₆H₄—ONa, NMP, 60° C.; i) SOP 8.

Compound E-5:

¹H-NMR (400 MHz, CDCl₃): δ=10.20 (dd, 1H, J_(NH,═C—H)=14.0 Hz, J_(NH,H-2)=9.9 Hz, NH), 8.80 (s, 1H, C═NH), 8.16 (d, 1H, ═C—H), 7.99 (m, 2H, Aryl) 7.58 (m, 1H, Aryl), 7.45 (m, 2H, Aryl), 7.30-7.17 (m, 10H, Aryl), 6.42 (d, 1H, J_(1,2)=3.6 Hz, H-1α), 4.89 (d, 1H, J_(gem)=8.4 Hz, OCH₂), 4.68-4.60 (m, 3H, OCH₂), 4.58 (dd, 1H, J_(5,6a)=2.0 Hz, J_(gem)=12.4 Hz, H-6a), 4.51 (dd, 1H, J_(5,6b)=4.0 Hz, H-6b), 4.22 (m, 1H, H-5), 4.03 (dd, 1H. J_(3,4)≈J_(2,3)=9.6 Hz, H-3), 3.80 (dd, 1H, J_(4,5)=9.4 Hz, H-4), 3.70 (ddd, 1H, H-2), 3.32 (s, 3H, NCH₃), 3.25 (s, 3H, NCH₃).

EXAMPLE 17 Preparation of L-iduronic Acid Containing Disaccharides B-A-1 to B-A-10

Example 17: Preparation L-iduronic acid containing disaccharides B-A-1 to B-A-10; a) SOP 32a, (80%, for B-A-1); b) SOP 27, (88%, for B-A-5); c) 1.SOP 20; 2. SOP 16, (84% for B-A-7, 2 steps); d) SOP 24, (94%, for B-A-9).

Formation of Disaccharide B-A-1 (Step a)

A suspension of A-1 (410 mg, 990 μmol), B-1 (680 mg, 990 μmol) and freshly activated molecular sieves 4 Å (1.0 g in dry CH₂Cl₂ (20 mL) was stirred for 90 min at 0° C. N-Iodosuccinimide (405 mg, 1.8 mmol) was added and stirring continued for 20 min. After addition of trifluoromethanesulfonic acid (10.6 μl, 119.7 μmol), the reaction mixture was further stirred until completion (from 0° C. to 25° C.) and quenched with aqueous NaHCO₃-solution (10%). The mixture was diluted with CH₂Cl₂ and filtered through a celite pad. The filtrate was washed with a 10% KHCO₃/Na₂S₂O₃ solution, water and saturated brine solution, dried over MgSO₄ and evaporated. Final purification was achieved by silica gel column chromatography. Yield: 730 mg (80%).

Formation of Disaccharide B-A-7 (Step c)

Disaccharide B-A-5 (1.00 g, 1.15 mol) was dissolved in anhydrous DMF (7.0 ml) and reacted with pyridinium dichromate (4.33 g, 11.5 mmol) under stirring at room temperature until complete conversion into the uronic acid. The reaction mixture was subsequently poured into 50 ml water and the whole extracted with diethyl ether. The combined ether layers were washed with 10% aqueous citric acid solution, filtered through a short silica gel pad, dried over MgSO₄, evaporated and dried under high vacuum. The crude residue was dissolved in Toluene (3 ml) and methanol (3 ml) and titurated with TMSCHN₂ solution (2M in hexane) until completion. The excess of TMSCHN₂ was destroyed by addition of acetic acid and the mixture evaporated. Final purification was achieved via silica gel chromatography. Yield: 871 mg (84%).

Compound B-A-9:

¹H-NMR (400 MHz, CDCl₃): δ=8.03 (m, 2H, Aryl), 7.91 (m, 2H, Aryl), 7.53 (m, 2H, Aryl), 7.42-7.23 (m, 14H, Aryl), 5.37 (d, 1H, J_(1,2)<1.5 Hz, H-1′α), 5.21 (m, 1H, H-2′), 4.97 (d, 1H, J_(4,5)=2.3 Hz, H-5′), 4.84 (d, 2H, J_(gem)=10.8 Hz, OCH₂), 4.81 (d, 1H, J_(gem)=10.8 Hz, OCH₂), 4.80 (d, 1H, J_(1,2)=3.6 Hz, H-1α), 4.77 (1H, J_(5,6a)=1.8 Hz, H-6a), 4.70 (m, 2H, OCH₂), 4.47 (dd, 1H, J_(5,6b)=4.2 Hz, J_(gem)=12.3 Hz, H-6b), 4.05-3.97 (m, 3H, H-4′, H-4, H-5), 3.91-3.87 (m, 2H, H-3′, H-3), 3.49 (s, 3H, OCH₃), 3.44 (m, 1H, H-2), 3.43 (s, 3H, OCH₃). Selected ¹³C-NMR (100 MHz, CDCl₃): δ=98.73 C-1 (J_(CH)=172.5 Hz), 98.35 C-1′ (J_(CH)=171.8 Hz).

EXAMPLE 18 Syntheses of Building Blocks E-D-1 to E-D-12

Example 18: Syntheses of disaccharides E-D-1 to E-D-12, conditions: a) SOP 32 a/b for X=SMe/Scres or SOP 33 for X=OTCA, (86% for E-D-1 via E-1, 84% for E-D-4 via E-5, as (α/β?mixtures), b) 1. SOP 9; 2. SOP 25a, (90% for E-D-7 over 2 steps).

Preparation of E-D-1 Methyl(2-azido-6-O-benzoyl-3,4-di-O-benzyl-2-deoxy-α-D-glucopyranosyl)-(1→4)-tert-butyldiphenylsilyl 2,3-di-O-benzyl-β-D-glucopyranosid)uronate.

A mixture of 2-azido-6-O-benzoyl-2,3 di-O-benzyl-2-deoxy-α/β-D-glucopyranosyl trichloroacemidate (2.5 g, 3.94 mmol), and methyl(tert-butyidiphenylsilyl 2,3-di-O-benzyl-β-D-glucopyranoside) uronate (1.6 g, 2.629 mmol) and molecular sieves 4A (2.5 g) in 50 ml diethyl ether was treated with TBDMSOTf (180 μl, 788.76 μmol) at −20° C. for 1 h. The reaction was quenched filtered, concentrated and the residue purified by silica gel column chromatography to obtain the desired disaccharide 2.48 g, 86%. R_(f)=0.67 (toluene/ethyl acetate 9/1).

Compound E-D-1:

E-D-1 was formed according to SOP 33 with ether as solvent at −30° C. and TBDMSOTf as promotor in 86% yield (α/β-mixture).

¹H-NMR (400 MHz, CDCl₃): δ=8.00 (m, 2H, Aryl), 7.68 (m, 4H, Aryl), 7.56 (m, 1H, Aryl), 7.42 (m, 4H, Aryl), 7.36-7.17 (m, 24H, Aryl), 5.47 (d, 1H, J_(1,2)=3.8 Hz, H-1′α), 5.02 (d, 1H, J_(gem)=11.4 Hz, OCH₂), 4.97 (d, 1H, J_(gem)=11.0 Hz, OCH₂), 4.84 (m, 4H, OCH₂), 4.75 (d, 1H, J_(gem)=11.4 Hz, OCH₂), 4.66 (d, 1H, J_(1,2)=7.5 Hz, H-1β), 4.57 (d, 1H, J_(gem)=10.9 Hz, OCH₂), 4.45 (m, 2H, H-6′a, H-6′b), 4.15 (dd, J=8.8 Hz and J=9.6 Hz), 3.86 (m, 1H), 3.65 (s, 3H, OCH₃, 3.68-3.58 (m, 3H), 3.55 (d, 1H, J_(4,5)=10.0 Hz, H-5), 3.31 (dd, 1H, J_(2,3)=10.2 Hz, H-2′), 1.12 (s, 9H, C(CH₃)₃).

Compound E-D-4:

E-D-4 was formed according to SOP 33 with ether as solvent at −30° C. and TBDMSOTf as promotor in 84% yield (α/β-mixture).

Selected ¹H-NMR (400 MHz, CDCl₃): δ=10.02 (dd, 1H, J_(NH,═CH)=14.4 Hz, J_(NH,H-2)=9.6 Hz, NH), 8.02 (m, 2H, Aryl), 7.79 (d, 1H, ═C—H), 7.72-6.93 (m, 33H, Aryl), 5.60 (d, 1H, J_(1,2)=3.6 Hz, H-1′α), 4.49 (d, 1H, J_(1,2)=7.8 Hz, H-1β), 3.66 (s, 3H, OCH₃), 3.29 (s, 3H, NCH₃), 3.28 (s, 3H, NCH₃), 1.14 (s, 9H, C(CH₃)₃).

Preparation of E-D-7: Methyl(2-azido-6-O-benzoyl-3,4-di-O-benzyl-2-deoxy-α-D-glucopyranosyl)-(1→4)-2,3-di-O-benzyl-β-D-glucopyranosyl trichloroacetimidyl)uronate.

A solution of methyl(2-azido-6-O-benzoyl-3,4-di-O-benzyl-2-deoxy-α-D-glucopyranosyl)-(1→4)-tert-butyldiphenylsilyl 2,3-di-O-benzyl-β-D-glucopyranoside) uronate (2.09 g, 1.903 mmol) in acetic acid (1.74 ml, 30.45 mmol) and 1 M solution of tetrabutylammoniumfluoride (7.6 ml, 7.61 mmol) was stirred at room temperature overnight. The reaction mixture was then concentrated and the residual syrup was purified by silica gel column chromatography to obtain the desired hemiacetal. Yield: 1.57 g (95.8%), R_(f)=0.21 (toluene/ethyl acetate 9/1).

A mixture of methyl(2-azido-6-O-benzoyl-3,4-di-O-benzyl-2-deoxy-α-D-glucopyranosyl)-(1→4)-2,3-di-O-benzyl-β-D-glucopyranosyl)uronate (594 mg, 690.76 μmol), trichloroacetonitrile (280 μl, 2.79 mmol) and DBU (31 μl, 209.3 μmol) in 8.0 ml dichloromethane was stirred at 0° C. for 1 h. The mixture was then concentrated and purified on a short column of silica gel to obtain the title compound as an amorphous white solid. Yield: 662 mg (95.3%), R_(f)=0.46 (toluene/ethyl acetate 9/1).

Compound E-D-7:

Selected ¹H-NMR (400 MHz, CDCl₃): δ=8.68 (s, 1H, C═NH), 8.00 (m, 2H, Aryl), 7.56 (m, 2H, Aryl), 7.43-7.23 (m, 22H, Aryl), 6.48 (d, 1H, J_(1,2)=4.3 Hz, H-1α), 5.59 (d, 1H, J_(1,2)=3.6 Hz, H-1′α), 5.03 (1H, J_(gem)=10.8 Hz, OCH₂), 4.93-4.83 (m, 4H, OCH₂), 4.70 (d, 1H, J_(gem)=12.0 Hz, OCH₂), 4.64 (d, 1H, J_(gem)=12.0 Hz, OCH₂), 4.60 (d, 1H, J_(gem)=11.2 Hz, OCH₂), 4.47 (m, 2H, H-6′a, H-6′b), 4.42 (m, 1H, not assigned), 4.15 (m, 2H, not assigned), 3.97 (dd, 1H, J=8.2 Hz and J=10.2 Hz, not assigned), 3.80 (m, 1H, not assigned), 3.76 (m, 3H, OCH₃), 3.72-3.64 (m, 2H, not assigned), 3.30 (dd, 1H, J_(2,3)=10.4 Hz, H-2′).

EXAMPLE 19 Syntheses of Disaccharides E-D-13 to E-D-44

Example 19: Syntheses of disaccharides E-D-13 to E-D-44, conditions: a) SOP 32 a/b for X=SMe/Scres or SOP 33 for X=OTCA (70% for E-D-23, α/β mixture); b) 1. SOP 9; 2. SOP 25a.

Compound E-D-27:

E-D-27 was formed according to SOP 33 with ether as solvent at −20° C. and TBDMSOTf as promotor in 70% yield (α/β-mixture).

Selected ¹H-NMR (400 MHz, CDCl₃): δ=7.58 (m, 2H, Aryl), 7.54 (m, 2H, Aryl), 7.36-7.00 (m, 23H, Aryl), 6.73 (m, 2H, Aryl), 5.37 (d, 1H, J_(1,2)=3.9 Hz, H-1′α), 5.12 (dd, 1H, J_(2,3)=8.8 Hz, H-2), 4.63 (d, 1H, J_(gem)=11.2 Hz, OCH₂), 4.58 (d, 1H, J_(gem)=11.2 Hz, OCH₂), 4.48 (d, 1H, J_(1,2)=7.3 Hz, H-1β), 3.66 (s, 3H, OCH₃), 3.55 (s, 3H, OCH₃), 3.34 (m, 1H), 3.22 (dd, 1H, J=3.4 Hz, J=10.7 Hz), 1.81 (s, 3H, OAc), 0.98 (s, 9H, C(CH₃)₃).

EXAMPLE 20 Synthesis of Alternative E-D-disaccharides E-D-45 to E-D-50

Example 20 Synthesis of alternative E-D-disaccharides E-D-45 to E-D-50, conditions: a) SOP 32a/b for X=SMe/SCres or SOP 33 for X=OTCA, diethyl ether, TBDMS-OTf, −20 deg. C (75% for E-D-45 as (α/β-mixture).

EXAMPLE 21 Synthesis of Trisaccharides E-D-C-1 to E-D-C-16

Example 21: Synthesis of trisaccharide E-D-C-1 to E-D-C-16, conditions: a) SOP 33, (70% for E-D-C 15 as an α/β mixture).

Compound E-D-C-15:

E-D-C-15 was formed according to SOP 33 with dichloromethane as solvent at 0 to 20° C. and TBDMSOTf as promotor in 70% yield (α/β-mixture).

¹H-NMR (400 MHz, CDCl₃): δ=7.93 (m, 2H, Aryl), 7.87 (m, 2H, Aryl), 7.66 (m, 2H, Aryl), 7.61 (m, 2H, Aryl), 7.46 (m, 2H, Aryl), 7.38-6.99 (m, 32H, Aryl), 6.79 (m, 2H, Aryl), 5.27 (d, 1H, J_(1,2)=3.8 Hz, H-1″α), 4.99 (dd, 1H, J_(3,4)≈J_(2,3)=9.5 Hz, H-3), 4.80-4.69 (m, 6H, OCH₂), 4.52 (m, 3H, OCH₂), 4.40 (d, 1H, J_(1,2)=8.0 Hz, H-1β), 4.38-4.32 (m, 2H, not assigned), 4.29 (d, 1H, J_(1,2)=7.5 Hz, H-1′β), 4.15 (m, 1H, J_(gem)=12.0 Hz, OCH₂), 4.02 (dd, 1H, J_(4,5)=9.6 Hz, H-4), 3.80 (2 dd, 2H, H-3″, H-4′), 3.71, (s, 3H, OCH₃), 3.67 (m, 1H, not assigned), 3.61-3.53 (m, 2H, H-5′, H-2′), 3.46 (dd, 1H, J_(gem)=11.2 Hz, J_(5,6a)=2.4 Hz, H-6a), 3.41 (dd, 1H, J_(2,3) ≈J _(3,4)=9.0 Hz, H-3′), 3.27 (s, 3H, OCH₃), 3.21 (dd, 1H, J_(2,3)=10.0 Hz, H-2″), 3.14 (dd; 1H, H-2′), 3.00 (dd, 1H, J_(5,6b)<2.0 Hz, H-6b), 2.75 (m, 1H, H-5) 1.05 (s, 9H,C(CH₃)₃).

EXAMPLE 22 Synthesis of Trisaccharides E-D-C-17 to E-D-C-32

Example 22: Synthesis of trisaccharide E-D-C-17 to E-D-C-32, conditions: a) SOP 33.

EXAMPLE 23 Formation of Trisaccharidic Trichloroacetimidates E-D-C-33 to E-D-C-48

Example 23: Formation of trisaccharidic Trichloroacetimidates E-D-C-33 to E-D-C-48, conditions: a) 1. SOP 9; 2. SOP 25, (82% over 2 steps for E-D-C-47

Example 24 Syntheses of Trisaccharides E-D-C-9 to E-D-C-12 and E-D-C-49 to E-D-C-60

Example 24: Syntheses of trisaccharides E-D-C-9 to E-D-C-12 and E-D-C-49 to E-D-C-60, conditions: a) 1. SOP 33; 2. SOP 24; b) SOP 33. (for D-C-5: 70%, 2 steps); b) SOP 33, (78% for E-D-C-9 as an α/β mixture).

Compound D-C-5:

D-C-5 was formed according to SOP 33 with ether as solvent at −20° C. and TMSOTf as promotor, followed by SOP 24 in 70% yield (over 2 steps as α/β-mixture).

Selected ¹H-NMR (400 MHz in CDCl₃): δ=7.88 (m, 2H, Ar), 7.67-7.58 (m, 5H, Ar), 7.42 (m, 2H, Ar), 7.37-7.12 (m, 16H, Aryl), 6.84 (m, 3H, Ar), 5.14 (dd, 1H, J_(1,2)=8.2 Hz, J_(2,3)=9.5 Hz, H-2′), 4.90 (d, 1H, J_(gem)=10.7 Hz, OCH₂), 4.73 (d, 1H; J_(gem)=11.5 Hz, OCH₂), 4.65 (d, 1H, J_(1,2)=8.2 Hz, H-1′β), 4.63-4.58 (m, 2H, OCH₂), 4.51 (d, 1H, J_(gem)=12.0 Hz, OCH₂), 4.20 (d, 1H, J_(1,2)=7.9 Hz, H-1β), 4.05 (d, 1H, J_(gem)=11.9 Hz, OCH₂), 4.02-3.95 (m, 2H, not assigned), 3.81 (s, 3H, OCH₃), 3.80 (s, 3H, OCH₃), 3.71 (d, 1H, J_(4,5)=9.9 Hz, H-5′), 3.67 (s, 3H, OCH₃), 3.47-3.40 (m, 3H, not assigned), 3.21 (dd, 1H, J=9.0 Hz, J=9.8 Hz, not assigned), 3.00 (dd, 1H, J_(5,6b)=1.4 Hz, J_(gem)=10.5 Hz, H-6b), 2.63 (m, 1H, H-5), 2.35 (bs, 1H, 4-OH), 1.07 (s, 9H, C(CH₃)₃).

Compound E-D-C-9:

E-D-C-9 was formed according to SOP 33 with ether as solvent at −20° C. and TBDMSOTf as promotor in 78% yield (α/β-mixture).

Selected ¹H-NMR (400 MHz, CDCl₃): δ=7.77 (m, 2H, Aryl), 7.59, 7.54 (2m, 2×2H, Aryl), 7.35-7.00 (m, 30H, Aryl), 6.88 (m, 2H, Aryl), 6.82 (m, 2H, Aryl), 6.73 (m, 2H, Aryl), 5.41 (d, 1H, J_(1,2)=3.5 Hz, H-1″a), 5.19 (dd, 1H, J_(2,3) ≈J_(1,2)=9.6 Hz, H-2′), 4.85-4.78 (m, 4H, OCH₂), 4.67 (m, 2H, OCH₂), 4.65 (d, 1H, J_(1,2)=8.5 Hz, H-1β, not assigned), 4.38 (d, 1H, J_(gem)=11.1 Hz, OCH₂), 4.29 (d, 1H, J_(gem)=11.7 Hz, OCH₂), 4.17 (dd, 1H, not assigned), 4.11 (d, 1H, J_(1,2)=7.9 Hz, H-1β not assigned), 4.03 (d, 1H, J_(gem)=12.0 Hz, OCH₂), 3.90-3.76 (m, 3H, not assigned), 3.730, 3.727 (2s, 2×3H, OCH₃), 3.65 (s, 3H, OCH₃), 3.54 (s, 3H, OCH₃), 2.89 (dd, 1H, J_(gem)=10.5 Hz, J_(5,6b)<2.0 Hz, H-6b), 2.52 (m, 1H, H-5), 1.02 (s, 9H, C(CH₃)₃).

EXAMPLE 25 Synthesis of Trisaccharides E-D-C-41, E-D-C-42 and E-D-C-61 to E-D-C-66

Example 25: Synthesis of trisaccharides E-D-C-41, E-D-C-42 and E-D-C-61 to E-D-C-66, conditions: a) 1. SOP 39; 2. SOP 38; 3. SOP 16; b) 1. SOP 14; 2. SOP 25a.

EXAMPLE 26 An Alternative Route to the Trisaccharides E-D-C-61 and E-D-C-63

Example 26 An alternative route to the trisaccharides E-D-C-61 and E-D-C-63, conditions: a) 1. SOP 39; 2. SOP 38; 3. SOP 16; 4. Pd(Ph₃P)₄, p-TolSO₂Na, THF, MeOH; b) SOP 33.

EXAMPLE 27 Syntheses of Blocks E-D-C-67 to E-D-C-70

Example 27: Syntheses of trisaccharides E-D-C-67 to E-D-C-70, conditions: a) SOP 33; b) SOP 24; c) SOP 33; d) 1. SOP 36; 2. SOP 37.

EXAMPLE 28 Synthesis of Trisaccharides E-D-C-70 and E-D-C-71

Example 28 Synthesis of trisaccharides E-D-C-70 and E-D-C-71, conditions: a) SOP 33, (55% for E-D-C-71, α/β mixture).

Compound E-D-C-71:

E-D-C-71 was formed according to SOP 33 with dichloromethane as solvent at 40° C. and TBDMSOTf as promotor in 55% yield (as α/β-mixture).

Selected ¹H-NMR (400 MHz, CDCl₃): δ=7.91 (m, 2H, Aryl), 7.61 (m, 2H, Aryl), 7.55 (m, 2H, Aryl), 7.50-7.02 (m, 29H, Aryl), 6.65 (m, 4H, Mp), 5.38 (d, 1H, J_(1,2)=3.9 Hz, H-1″α), 5.22 (bs, 1H, NH), 4.67 (d, 1H, J_(1,2)=7.4 Hz, H-1β, not assigned), 4.50 (d, 1H, J_(1,2)=7.8 Hz, H-1β, not assigned), 3.92 (d, 1H, J_(4,5)=9.8 Hz, H-5′), 3.698 (s, 3H, OCH₃), 3.693 (s, 3H, OCH₃), 1.03 (s, 9H, C(CH₃)₃).

M_(found)=1408.52 (M+H₂O)⁺, M_(calc), =1390.54 (M⁺).

EXAMPLE 29 Syntheses of Trisaccharides E-D-C-61, E-D-C-72 and E-D-C-73

Example 29: Syntheses of trisaccharides E-D-C-72 to E-D-C-73 and E-D-C-61, conditions: a) SOP 33; b) SOP 24; c) SOP 33.

EXAMPLE 30 Syntheses of Trisaccharides C-B-A-1 to C-B-A-4

Example 30 Syntheses of trisaccharides C-B-A-1 to C-B-A-4, conditions: a) SOP 33; b) SOP 32a; c) 1. SOP 27; 2. SOP 20; 3. SOP 16; 4. SOP 24.

EXAMPLE 31 Synthesis of Trisaccharides C-B-A-5 to C-B-A-8

Example 31: Synthesis of trisaccharides C-B-A-5 and C-B-A-8, conditions: a) SOP 33, (50% for C-B-A-5, α/β mixture).

Compound C-B-A-5:

C-B-A-5 was formed according to SOP 33 with ether as solvent at −20° C. and TBDMSOTf as promotor in 50% yield (as α/β-mixture). M_(found)=1269.65 (M+H+H₂O)⁺, M_(calc)=1250.43 (M⁺).

EXAMPLE 32 Syntheses of D-C-B Trisaccharides D-C-B-1 to D-C-B-3.

Example 32: Syntheses of D-C-B-trisaccharides D-C-B-1 to D-C-B-3, conditions: a) 1. SOP 33; 2. SOP 36; 3. SOP 37.

EXAMPLE 33 Syntheses of D-C-B Trisaccharides D-C-B4 to D-C-B-7.

Example 33 Syntheses of D-C-B-trisaccharides D-C-B-4 to D-C-B-7, conditions: a) 1. SOP 33; 2. SOP 36; 3. SOP 37.

EXAMPLE 34 Syntheses of Tetrasaccharides D-C-B-A-1 to D-C-B-A-2

Example 34: Syntheses of tetrasaccharides D-C-B-A-1 and D-C-B-A-2, conditions: a) SOP 32a; b)1. SOP 36; 2. SOP 37; 3. SOP 24.

EXAMPLE 35 Alternative Syntheses of Tetrasaccharides D-C-B-A-2

Example 35: Alternative synthesis of tetrasaccharide D-C-B-A-2, conditions: a) 1. SOP 34; 2. SOP 24.

EXAMPLE 36 Syntheses of Tetrasaccharides D-C-B-A-3 to D-C-B-A-8

Example 36: Syntheses of tetrasaccharides D-C-B-A-3 to D-C-B-A-8, conditions: a) 1 SOP 32b; 2. SOP 24.

EXAMPLE 37 Syntheses of Tetrasaccharides E-D-C-B-1 to E-D-C-B-4

Example 37: Syntheses of tetrasaccharides E-D-C-B-1 to E-D-C-B-4, conditions: a) SOP 33; b) 1. SOP 36; 2. SOP 37.

EXAMPLE 38 Syntheses of Blocks E-D-C-B-5 to E-D-C-B-8

Example 38: Syntheses of tetrasaccharides E-D-C-B-5 to E-D-C-B-8, conditions: a) SOP 33; b) 1. SOP 36; 2. SOP 37.

EXAMPLE 39 Syntheses of E-D-C-B-A Pentasaccharides P-1 and P-2

Example 39: Syntheses of E-D-C-B-A pentasaccharides P-1 and P-2, conditions: a) SOP 34.

EXAMPLE 40 Synthesis of E-D-C-B-A Pentasaccharides P-3 to P-26

Example 40: Synthesis of E-D-C-B-A pentasaccharide P-3 to P-26, conditions: a) SOP 33 (75% for P-19 as an α/β mixture).

Compound P-19:

P-19 was formed according to SOP 33 with dichloromethane as solvent at −20° C. and TMSOTf as promotor;

M_(found)=2068.76 (M+H+H₂O)⁺, M_(calc)=2049.74 (M⁺).

EXAMPLE 41 Alternative Syntheses of E-D-C-B-A Pentasaccharides P-11, P-12, P-19, P-20 and P-27

Example 41: Alternative syntheses of E-D-C-B-A pentasaccharides P-11, P-12, P-19, P-20 and P-27, conditions: a) SOP 32a.

EXAMPLE 42 Alternative Syntheses of Some E-D-C-B-A Pentasaccharides

Example 42: Alternative syntheses of some E-D-C-B-A pentasaccharides, conditions: a) SOP 32a (for R=SCres) or SOP 33 (for R=OTCA).

EXAMPLE 43 Synthesis of Pentasaccharide P-13 and P-30

Example 43: Formation of pentasaccharides P-13 and P-30, conditions: a) SOP 32a.

EXAMPLE 44 Formation of Pentasaccharide P-19 and P-31

Example 45 Partial deprotection of pentasaccharide P-19, conditions: a) SOP 28, 84%; b) SOP 39, 86%.

Example Preparation of P19:

A mixture of O-(2-azido-6-O-benzoyl-3,4-di-O-benzyl-2-deoxy-α-D-glucopyranosyl)-(1→4)-(methyl 2,3-di-O-benzyl-β-D-glucopyranosyluronate)-(1→4)-2-azido-3-O-benzoyl-2-deoxy-6-O-p-methoxybenzyl-α-D-glucopyranosyltrichloroacetimidate (30.0 mg, 21.2 μmol) and methyl(methyl 2-O-benzoyl-3-O-benzyl-α-L-idupyranosyluronate)-(1→4)-2-azido-3-O-benzyl-6-O-benzoyl-2-deoxy-α-D-glucopyranoside (15.4 mg 19.3 μmol) and 100 mg of molecular sieves 4 Å in 1.5 ml dry dichloromethane was treated with TBDMSOTf (0.97 μl, 4.24 μmol) at −20° C. for 20 hours. The reaction was quenched, filtered and concentrated. Further purification of the title compound was achieved by silica gel chromatography Yield: 15.83 mg (40%), R_(f)=0.30 (toluene/ethyl acetate=9/1).

EXAMPLE 45 Partial Deprotection of Pentasaccharide P-19

Example 45: Partial deprotection of pentasaccharide P-19, conditions: a) SOP 28, 84%; b) SOP 39, 86%.

Compound P-33:

M_(found)=1503.5 (M−N₂+2H)⁺, M_(calc)=1529.51 (M⁺).

To ease the structural proof, a small part of P-33 was transformed into the bis methyl uronurate derivative and characterized via NMR-spectroscopy. Characteristic ¹H-NMR-spectral regions are shown in FIG. 1.

M_(found)=1514.62 (M+H)⁺, M_(calc)=1513.58 (M⁺).

EXAMPLE 46 Partial Deprotection of Pentasaccharide P-30, Containing a DTPM-Group as Amino Protection

Example 46 Partial deprotection of pentasaccharide P-30, containing a DTPM-group as amino protection, conditions: a) 1. SOP 28; 2. SOP 39; b) 1. SOP 11 with MeNH₂ as primary amine and MeOH as solvent; 2. SOP 12.

EXAMPLE 47 Partial Deprotection of Pentasaccharide P-1, Containing a Cyclic Carbamate as Amino Protection

Example 47: Partial deprotection of pentasaccharide P-1, containing a cyclic carbamate as amino protection, conditions: a) SOP 27; b) SOP 39; c) SOP 12.

EXAMPLE 48 Deprotection Protocol for Pentasaccharides P-37 of Claim 4

Example 48: Deprotection protocol for pentasaccharides P-37 of claim 4, conditions: a) 1. SOP 27 and 28; 2. SOP 39; 3. SOP 11 with MeNH₂ as primary amine and MeOH as solvent; 4. SOP 12.

EXAMPLE 49 Transformation of Pentasaccharide P-33 into the O- and N-Sulfated Pentasaccharide P-35

Example 49: Transformation of pentasaccharide P-33 into the sand N-sulfated pentasaccharide P40, conditions: a) SO₃xNMe₃, DMF, 50° C.; b) Fb (70 psi), Pd/C, H₂O; c) SO₃xPyridine, H₂O, pH=9.5. The transformation of P-33 into P40 has been performed according to literature: Petitou et al., Carbohydr. Res. 1987, 167, 67-75.

The ¹H-NMR (400 MHz, D₂O) of P40 is shown in FIG. 2. 

1. A pentasaccharide building block for the preparation of synthetic heparinoids, said building block being of General Formula I,

In which the configuration of the monosaccharidic units and the stereochemistry of the internal linkages is defined as D-gluco-alpha-1,4-D-glucurono-beta-1,4-D-Gluco-alpha-1,4-L-idurono-beta-1,4-D-gluco, and the substituents are defined as; X₁ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido, NH-Ac, NH-Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl.
 2. The pentasaccharide building block of claim 1, wherein the configuration of the monosacharidic units and the stereochemistry of the internal linkages is defined as D-gluco-alpha-1,4-D-glucurono-beta-1,4-D-Gluco-alpha-1,4-L-idurono-beta-1,4-D-gluco, wherein: X₁ is selected from the group consisting of hydroxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H), R_(H1), and R_(H2) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido; or other such suitable protected amino functions or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1), R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, benzoyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(B) and R_(B1) are independently selected from an azido function, an amine; an NH-Dde or NH-DTPM group; additionally R_(S4) and R_(B) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(P1) and R_(P2) are independently selected from benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl, and alkylarylacyl protecting groups; and carbonate protecting groups.
 3. The pentasaccharide building block of claim 1, wherein X₁ is selected from the group consisting of hydroxy, methoxy, ethoxy, allyloxy, n-pentenyloxy, C₁₋₅ alkoxy, C₃₋₇ alkenyloxy, benzyloxy, substituted benzyloxy; thiomethyl, thioethyl, thiocresyl, thiophenyl, chloro, bromo, fluoro, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy, 2-trimethylsilyloxyethoxy (SEM), or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido, NH-Ac, NH-Alloc; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; tert-butyldiphenylsilyl, allyl, tert-butyldimethylsilyl, methoxymethyl, methoxyethyl, trimethylsiloxyethyl (SEM), benzyloxymethyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; tert-butyldiphenylsilyl, allyl, tert-butyldimethylsilyl, methoxymethyl, methoxyethyl, trimethylsiloxyethyl, benzyloxymethyl; R_(S3) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; tert-butyldiphenylsilyl, allyl, tert-butyldimethylsilyl, methoxymethyl, methoxyethyl, trimethylsiloxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; tert-butyldiphenylsilyl, allyl, tert-butyldimethylsilyl, methoxymethyl, methoxyethyl, trimethylsiloxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; tert-butyldiphenylsilyl, allyl, tert-butyldimethylsilyl, methoxymethyl, methoxyethyl, trimethylsiloxyethyl, benzyloxymethyl; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(P1) is selected from the group consisting of benzyl; 4-methoxybenzyl, substituted benzyl groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; R_(P2) is selected from the group consisting of benzyl; 4-methoxybenzyl, substituted benzyl groups; benzoyl, 4-chlorobenzoyl, chloroacetyl, acetyl, levulinoyl, pivaloyl, 4-methoxybenzoyl, 4-azidobenzoyl, alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups.
 4. The pentasaccharide building block of claim 1, wherein the configuration of the monosacharidic units and the stereochemistry of the internal linkages is defined as D-gluco-alpha-1,4-D-glucurono-beta-1,4-D-Gluco-alpha-1,4-L-idurono-beta-1,4-D-gluco, and wherein: X₁ is selected from the group consisting of hydroxy, methoxy, thiomethyl, thioethyl, thiocresyl, thiophenyl, trichloroacetimidoyl, a ^(t)butyldiphenylsilyloxy a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H), R_(H1), and R_(H2) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1), R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; benzoyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, allyl, benzyl or substituted benzyl groups; R_(B) and R_(B1) are independently selected from an azido function; additionally R_(S4) and R_(B) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoyl, allyloxycarbonyl.
 5. The pentasaccharide building block of claim 1, wherein X₁ is C₁ to C₅ alkoxy.
 6. The pentasaccharide building block of claim 1, wherein R_(A) is azido or R_(H1) and R_(A) can combine together to form a cyclic carbamate.
 7. The pentasaccharide building block of claim 4, wherein R_(P1), R_(H) and R_(H1) is benzyl.
 8. The pentasaccharide building block of claim 1, wherein R_(S1), R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from benzoyl, 4-methoxybenzyl, 4-methoxyphenyl, 4-chlorobenzoyl, or tert-butyldiphenylsilyl, allyl, trimethylsiloxyethyl.
 9. The pentasaccharide building block of claim 4, wherein R_(P1) and R_(P2) is benzyl or substituted benzyl.
 10. The pentasaccharide building block of claim 4, wherein R_(E2) is methyl.
 11. The pentasaccharide building block of claim 1, wherein R_(B) is azido or R_(B) and R_(S4) combine to form a cyclic carbamate.
 12. The pentasaccharide building block of claim 1, wherein R_(S1), and R_(S2) is benzoyl, and R_(A) is azido or R_(A) and R_(H1) together combine to form a cyclic carbamate.
 13. The pentasaccharide building block of claim 1, wherein R_(P2) is selected from benzyl, chloroacetyl, acetyl, benzoyl, substituted benzoyl, pivaloyl, levulinyl or allyloxycarbonyl.
 14. The pentasaccharide building block of claim 4, wherein R_(B1) is azido or R_(H2) and R_(B1) can combine together to form a cyclic carbamate.
 15. The pentasaccharide building block of claim 1, wherein X₁ is C₁-C₅ alkoxy, R_(S1) and R_(S2) is benzoyl, R_(S3), R_(S4) and R_(S5) are independently selected from benzoyl, 4-methoxybenzyl, 4-methoxyphenyl, 4-chlorobenzoyl, tert-butyldiphenylsilyl, allyl, R_(E2) is methyl, R_(P1), R_(H), R_(H1) and R_(H2) is benzyl and R_(P2) is selected from benzyl, chloroacetyl, acetyl, benzoyl, substituted benzoyl, pivaloyl, levulinyl or allyloxycarbonyl, R_(A) R_(B) and R_(B1) are independently selected from azido or any of (R_(A) and R_(H1)), or (R_(B) and R_(S4)) or (R_(B1) and R_(H2)) can combine together to form a cyclic carbamate.
 16. A method of synthesizing the pentasaccharide of claim 1, comprising the steps of forming a trisaccharide EDC of formula II,

wherein X₂ is selected from thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, n-pentenyl and the stereochemistry may be alpha or beta; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting groups, allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, carbamate protecting groups, trityl, or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; and forming a disaccharide BA of formula III

wherein: X₁ is selected from the group consisting of alkoxy, alkenyloxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Cbz, NH-Troc, N-phthalimido, NH-Alloc, NH-Ac; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(Q) is either a) —C═O)—OR_(E2) wherein R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; or b) —CH₂)—OR_(M) wherein R_(M) is selected from p-methoxyphenyl, p-methoxybenzyl, a trityl group, allyl, levulinoyl; R_(L) is H; and linking the trisaccharide to the disaccharide to form the pentasaccharide.
 17. A method of claim 16, wherein: X₁ is selected from the group consisting of hydroxy, methoxy, thiomethyl, thioethyl, thiocresyl, trichloroacetimidoyl, a ^(t)butyldiphenylsilyloxy a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H), R_(H1), and R_(H2) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate, R_(S1), R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; benzoyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, allyl or, benzyl and substituted benzyl groups; R_(B) and R_(B1) are independently selected from an azido function; an NH-Dde or NH-DTPM group; additionally R_(S4) and R_(B) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoyl, allyloxycarbonyl. X₂ is selected from thiomethyl, thiocresyl, or trichloroacetimidoyl, and the stereochemistry may be alpha or beta, R_(Q) is either a) —C═O)—OR_(E2) wherein R_(E2) is methyl, allyl or benzyl; or b) —CH₂)—OR_(M) wherein R_(M) is selected from p-methoxyphenyl, p-methoxybenzyl, a trityl group, R_(L) is H, and linking the trisaccharide to the disaccharide to form the pentasaccharide.
 18. A method of synthesizing the pentasaccharide of claim 1, comprising the step of synthesizing a tetrasaccharide EDCB (formula IV),

wherein: X₂ is selected from a thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, and the stereochemistry may be alpha or beta; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; and linking the tetrasaccharide to a monosaccharide of formula V

wherein: X₁ is selected from alkoxy, aryloxy, benzyloxy, substituted benzyloxy; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Cbz, NH-Troc, N-phthalimido, NH-Ac, NH-Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate, R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl.
 19. The method of claim 18 wherein: X₁ is selected from the group consisting of hydroxy, methoxy, thiomethyl, thioethyl, thiocresyl, trichloroacetimidoyl, a ^(t)butyldiphenylsilyloxy a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H), R_(H1), and R_(H2) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate, R_(S1), R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; benzoyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, allyl benzyl or substituted benzyl groups; R_(B) and R_(B1) are independently selected from an azido function; an NH-Dde or NH-DTPM group; additionally R_(S4) and R_(B) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoyl, allyloxycarbonyl; X₂ is selected from thiomethyl, thiocresyl, or trichloroacetimidoyl, and the stereochemistry may be alpha or beta, R_(E2) is methyl, allyl or benzyl;
 20. A method of synthesizing the pentasaccharide of claim 1, comprising synthesizing a tetrasaccharide DCBA of formula VI

X₁ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta, R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Cbz, NH-Troc, N-phthalimido, NH-Ac, NH-Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate, R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; benzyl or substituted benzyl groups, R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate, R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; and linking the tetrasaccharide to a monosaccharide of formula VII

wherein X₂ is selected from a thioalkyl, thioaryl, trichloroacetimidoyl; and the stereochemistry may be alpha or beta; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a _(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate.
 21. The method of claim 20, wherein: X₁ is selected from the group consisting of hydroxy, methoxy, thiomethyl, thioethyl, thiocresyl, trichloroacetimidoyl, a ^(t)butyldiphenylsilyloxy a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H), R_(H1), and R_(H2) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate, R_(S1), R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; benzoyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, allyl or, benzyl and substituted benzyl groups; R_(B) and R_(B1) are independently selected from an azido function; an NH-Dde or NH-DTPM group; additionally R_(S4) and R_(B) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoyl, and allyloxycarbonyl; X₂ is selected from thiomethyl, thiocresyl, or trichloroacetimidoyl, and the stereochemistry may be alpha or beta; R_(E2) is methyl, allyl or benzyl.
 22. A method of synthesizing the pentasaccharide of claim 1, comprising the steps of forming a disaccharide ED of formula VIII,

wherein X₂ is selected from thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, and the stereochemistry may be alpha or beta; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate. R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups, R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₃ alkenyl; and forming a trisaccharide CBA of formula IX

wherein X₁ is selected from the group consisting of alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta with the proviso that when X₁ is a halogen, trichloroacetimidoyl, phosphate or related phosphate ester type leaving group, X₂ may not be the same; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Cbz, NH-Troc, N-phthalimido, NH-Ac, NH-Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; and linking the disaccharide to the trisaccharide.
 23. The method of claim 22, wherein: X₁ is selected from the group consisting of hydroxy, methoxy, thiomethyl, thioethyl, thiocresyl, trichloroacetimidoyl, a ^(t)butyldiphenylsilyloxy a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H), R_(H1), and R_(H2) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate, R_(S1), R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; benzoyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, allyl or, benzyl and substituted benzyl groups; R_(B) and R_(B1) are independently selected from an azido function; an NH-Dde or NH-DTPM group; additionally R_(S4) and R_(B) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoyl, allyloxycarbonyl; X₂ is selected from thiomethyl, thiocresyl, or trichloroacetimidoyl, and the stereochemistry may be alpha or beta; R_(E2) is methyl, allyl or benzyl.
 24. A pentasaccharide of claim 1, wherein X₁ is selected from the group consisting of alpha or beta thiomethyl or thiocresyl or trichloroacetimidoyl or t-butyldiphenylsilyloxy, alpha methoxy; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl.
 25. A pentasaccharide of claim 1, wherein: X₁ is selected from the group consisting of alpha or beta thiomethyl or thiocresyl or trichloroacetimidoyl or t-butyldiphenylsilyloxy, alpha methoxy; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl; R_(H) is selected from the group consisting of benzyl; R_(S2) is selected from the group consisting of benzoyl, substituted arylacyl protecting groups; R_(E2) is methyl or allyl or benzyl.
 26. A pentasaccharide of claim 1, wherein: X₁ is selected from the group consisting of alpha or beta thiomethyl or thiocresyl or trichloroacetimidoyl or t-butyldiphenylsilyloxy, alpha methoxy; R_(S3) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl, allyl, allyloxycarbonyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl, allyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate.
 27. A pentasaccharide of claim 1, wherein: X₁ is selected from the group consisting of alpha or beta thiomethyl or thiocresyl or trichloroacetimidoyl or t-butyldiphenylsilyloxy, alpha methoxy; R_(E1) is selected from the group consisting of methyl, allyl, benzyl; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoate; or allyloxycarbonyl.
 28. The pentasaccharide of claim 1, wherein: X₁ is selected from the group consisting of alpha or beta thiomethyl or thiocresyl or trichloroacetimidoyl or t-butyldiphenylsilyloxy, alpha methoxy; R_(H) is benzyl; R_(H2) is benzyl; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, tert-butyldiphenylsilyl; R_(B1) is selected from the group consisting of an azido function, an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate.
 29. A monosaccharide of General Formula X

In which the ring is of the D-Gluco stereochemistry, wherein X₁ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, chloro, fluoro, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido, NH Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups; or R_(L) and R_(S1) can combine to form a benzylidene or substituted benzylidene ring.
 30. The monosaccharide of claim 29, wherein X₁ is selected from the group consisting of hydroxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, chloro, fluoro, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H1) is selected from group consisting of benzyl or substituted benzyl protecting group; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; benzoyl, arylacyl or alkylarylacyl, 4-chlorobenzoyl, allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; or R_(L) and R_(S1), can combine to form a benzylidene or substituted benzylidene ring.
 31. The monosaccharide of claim 29, wherein: X₁ is selected from the group consisting of alpha or beta thiomethyl or thiocresyl or trichloroacetimidoyl or t-butyldiphenylsilyloxy, alpha methoxy; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl; R_(L) is a H atom or levulinoyl.
 32. The monosaccharide of claim 31, wherein: R_(A) is azido or R_(A) and R_(H1) combine to form a cyclic carbamate.
 33. The monosaccharide of claim 31, wherein R_(H1) is benzyl.
 34. The monosaccharide of claim 31, wherein R_(S1) is selected from benzoyl, 4-methoxybenzyl, 4-methoxyphenyl, 4-chlorobenzoyl.
 35. A monosaccharide of General Formula XI,

In which the ring is of the L-Ido stereochemistry; wherein, R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups; X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta.
 36. The monosaccharide of claim 35, wherein, R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(S2) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group; X₂ is selected from C₁ to C₆ thioalkyl, or C₇ to C₁₂ thioaryl, and the stereochemistry may be alpha or beta.
 37. The monosaccharide of claim 35, wherein, R_(H) is selected from the group consisting of benzyl; R_(S2) is selected from the group consisting of benzoyl, substituted arylacyl protecting groups; R_(E2) is methyl, allyl or benzyl; R_(L) is selected from a H atom; or a levulinoyl; X₂ is selected from a t-butyldiphenylsiloxy, trichloroacetimidoyl, and the stereochemistry may be alpha or beta.
 38. The monosaccharide of claim 35, wherein, R_(H) is selected from the group consisting of benzyl; R_(S2) is selected from the group consisting of benzoyl, substituted arylacyl protecting groups; R_(E2) is methyl, allyl or benzyl; R_(L) is selected from a H atom; a levulinoyl; X₂ is selected from a thiomethyl or thiocresyl, and the stereochemistry may be alpha or beta.
 39. A monosaccharide of General Formula XII,

wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups; R_(M) is selected from a p-methoxyphenyl or p-methoxybenzyl protecting group or other suitable oxidatively labile protecting group; a trityl group; or R_(M) and R_(L) are combined together to form an cyclic acetal or ketal.
 40. The monosaccharide of claim 39, wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, trichloroacetimidoyl, and the stereochemistry may be alpha or beta; R_(S2) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; R_(H) is benzyl or a substituted benzyl protecting group; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group; R_(M) is selected from a p-methoxyphenyl or p-methoxybenzyl protecting group or other suitable oxidatively labile protecting group; a trityl group; or R_(M) and R_(L) are combined together to form an isopropylidene, benzylidene, 4-methoxybenzylidene, substituted benzylidene, cyclohexylidene or other alkylidene protecting group.
 41. The monosaccharide of claim 39, wherein: X₂ is selected from a thiomethyl or thiocresyl and the stereochemistry may be alpha or beta; R_(S2) is benzoyl; R_(H) is benzyl; R_(L) is selected from a H atom or a levulinoyl; R_(M) is selected from a p-methoxyphenyl or p-methoxybenzyl protecting group; or R_(M) and R_(L) are combined together to for an isopropylidene.
 42. The monosaccharide of claim 39, wherein: X₂ is selected from a trichloroacetimidoyl or t-butyldiphenylsilyloxy and the stereochemistry may be alpha or beta; R_(S2) is benzoyl; R_(H) is benzyl; R_(L) is selected from a H atom or a levulinoyl; R_(M) is selected from a p-methoxyphenyl or p-methoxybenzyl protecting group; or R_(M) and R_(L) are combined together to form an isopropylidene.
 43. A monosaccharide of General Formula XIII,

In which the ring is of the D-Gluco stereochemistry; Wherein: R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups; X₃ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta.
 44. A monosaccharide of claim 43, wherein: R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group; X₃ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta.
 45. The monosaccharide of claim 43, wherein: R_(S3) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl, allyl, allyloxycarbonyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl, allyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(B) is selected from the group consisting of an azido function, an amine, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(L) is selected from a H atom or a levulinoyl group; X₃ is selected from a thiomethyl, thioethyl, thiophenyl, thiocresyl, trichloroacetimidoyl, tert-butyldiphenylsilyloxy and the stereochemistry may be alpha or beta.
 46. A monosaccharide of General Formula XIV,

In which the ring is of the D-Gluco stereochemistry Wherein, R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; X₄ is selected from a hydroxyl group; thioalkyl, thioaryl, chloro, fluoro, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups.
 47. The monosaccharide of claim 46, wherein, R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; X₄ is selected from a hydroxyl group; thioalkyl, thioaryl, chloro, fluoro, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(L) is selected from a H atom; a levulinoyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group.
 48. The monosaccharide of claim 46, wherein, R_(E1) is selected from the group consisting of methyl, allyl, benzyl; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoate; or allyloxycarbonyl; X₄ is selected from tertbutyldiphenylsilyloxy, trichloroacetimidoyl or fluoro and the stereochemistry may be alpha or beta; R_(L) is selected from a H atom; a levulinoyl, pivaloyl.
 49. The monosaccharide of claim 46, wherein: R_(E1) is selected from the group consisting of methyl, allyl, benzyl; R_(P1) is benzyl R_(P2) is selected from the group consisting of benzyl; benzoate; or allyoxycarbonyl; X₄ is selected from a thiomethyl or thiocresyl and the stereochemistry may be alpha or beta; R_(L), is selected from a H atom; a levulinoyl, pivaloyl, benzoyl, allyl.
 50. A monosaccharide of General Formula XV.

In which the ring is of the D-Gluco stereochemistry; wherein: R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; X₅ is selected from a hydroxyl group; thioalkyl, thioaryl, chloro, fluoro, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta.
 51. The monosaccharide of claim 50, wherein: R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl; and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl; R_(B1) is selected from the group consisting of an azido function, an amine; NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; X₅ is selected from a hydroxyl group; thioalkyl, thioaryl, chloro, fluoro, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting groups; thiomethyl, and the stereochemistry may be alpha or beta.
 52. The monosaccharide of claim 50, wherein: R_(H) is benzyl R_(H2) is benzyl; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, tert-butyldiphenylsilyl; R_(B1) is an azido function, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; X₅ is selected from a thiomethyl, thiocresyl, trichloroacetimidate and the stereochemistry may be alpha or beta, with the proviso that X₅ may not be trichloroacetimidate when all of R_(B1) is azido and R_(H2) is unsubstituted benzyl and R_(S5) is unsubstituted benzoyl.
 53. A monosaccharide of General Formula XVI,

wherein: X₁ is selected from the group consisting of hydroxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups. R_(S6) is selected from the group consisting of 4-methoxyphenyl, 4-methoxybenzyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl; R_(L) and R_(S6) may also together combine to form an alkylidene, isopropylidene, benzylidene or substituted benzylidene ring.
 54. The monosaccharide of claim 53, wherein: X₁ is selected from the group consisting of hydroxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy, thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group, a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-Ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-FMoc-butyryl, 4-N-Boc-butyryl type protecting groups; R_(S6) is selected from the group consisting of 4-methoxyphenyl, 4-methoxybenzyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl; R_(L) and R_(S6) may also together combine to form an alkylidene, isopropylidene, benzylidene or substituted benzylidene ring.
 55. The monosaccharide of claim 53, wherein X₁ is selected from the group consisting of thiomethyl, thiocresyl, trichloroacetimidoyl, or a ^(t)butyldiphenylsilyloxy and the stereochemistry may be alpha or beta; R_(L) is selected from a H atom; a levulinoyl. R_(S6) is selected from the group consisting of 4-methoxyphenyl, 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl, or tert-Butyldiphenylsilyl; R_(L) and R_(S6) may also together combine to form an isopropylidene, benzylidene or 4-methoxybenzylidene ring;
 56. A disaccharide of formula XVII

wherein X₁ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido, NH-Ac, NH-Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, benzoyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups.
 57. The disaccharide of claim 56, wherein: X₁ is selected from the group consisting of hydroxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc N-phthalimido; or other such suitable protected amino functions, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; C3 to C6 alkylacyl, benzoyl; arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups, tert-Butyldiphenylsilyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-Ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group.
 58. The disaccharide of claim 56, wherein: X₁ is selected from the group consisting of alpha or beta thiomethyl or thiocresyl or trichloroacetimidoyl or t-butyldiphenylsilyloxy, alpha methoxy; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, R_(A) is an azido function, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl; R_(S2) is selected from the group consisting of benzoyl and substituted arylacyl; protecting groups; R_(H) is selected from the group consisting of benzyl; R_(S2) is selected from the group consisting of benzoyl and substituted arylacyl protecting groups; R_(E2) is methyl, allyl or benzyl; R_(L) is selected from a H atom; a levulinoyl.
 59. A disaccharide building block, of claim 58 wherein said building block is the General Formula XVIII,

wherein: X₁ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido, NH-Ac, NH-Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl; benzyloxymethyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(M) is selected from a p-methoxyphenyl or p-methoxybenzyl protecting group or other suitable oxidatively labile protecting group; a trityl group; or R_(M) and R₁ are combined together to form an isopropylidene, benzylidene, substituted benzylidene, cyclohexylidene or other acetal or ketal protecting group, R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups.
 60. The disaccharide building block, of claim 59, wherein: X₁ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy, thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports, and the stereochemistry may be alpha or beta; R_(A) is selected from the group consisting of an azido function, an NH-Dde, NH-DTPM, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; R_(S2) is selected from the group consisting of 4-methoxyphenyl, substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(M) is selected from a p-methoxyphenyl or p-methoxybenzyl protecting group or a trityl group; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group.
 61. The disaccharide building block, of claim 59, wherein, X₂ is selected from the group consisting of alpha or beta thiomethyl or thiocresyl or trichloroacetimidoyl or t-butyldiphenylsilyloxy; or alpha methoxy; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(A) is an azido function or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl; R_(H) is benzyl; R_(S2) is selected from the group consisting of benzoyl, substituted arylacyl protecting groups; R_(E2) is methyl, allyl or benzyl; R_(L) is selected from a H atom; a levulinoyl. R_(M) is selected from a p-methoxyphenyl or p-methoxybenzyl protecting group or a trityl group.
 62. A disaccharide building block of General Formula XIX,

wherein, X₂ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, chloro fluoro, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl, or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(B) is selected from the group consisting of an azido function, an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of hydroxy, 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups.
 63. The disaccharide building block of claim 62, wherein: X₂ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thiomethyl, thioethyl, thiocresyl, thiophenyl, chloro, fluoro, trichloracetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(S3) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, substituted benzyl groups, substituted arylacyl protecting groups, allyloxycarbonyl, 9-fluorenylmethoxycarbonyl, carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(B) is selected from the group consisting of an azido function, an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of hydroxy, 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group;
 64. The disaccharide building block of claim 62, wherein: R_(S3) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl, allyl, allyloxycarbonyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl, 4-methoxybenzyl, benzoyl, 4-chlorobenzoyl, allyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(B) is an azido function, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; X₃ is selected from a thiomethyl, thioethyl, thiophenyl, thiocresyl, trichloroacetimidoyl, tert-butyldiphenylsilyloxy and the stereochemistry may be alpha or beta; R_(E1) is selected from the group consisting of methyl, allyl, benzyl; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl, benzoate; or allyloxycarbonyl; R_(L) is selected from a H atom; a levulinoyl, pivaloyl, benzoyl, allyl;
 65. A disaccharide building block of General Formula XX

wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C3-C5 alkenyl, R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl, 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety.
 66. The disaccharide building block of claim 65, wherein: X₂ is selected from a hydroxyl group, thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(B1) is selected from the group consisting of an azido function, an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate. R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(S5) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups, C₃ to C₆ alkylacyl, C₆ to C₁₃ arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups, tert-Butyldiphenylsilyl.
 67. The disaccharide building block of claim 65 wherein: X₂ is selected from tertbutyldiphenylsilyloxy, trichloroacetimidoyl or fluoro; and the stereochemistry may be alpha or beta; R_(E1) is selected from the group consisting of methyl, allyl, benzyl; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl benzoate or allyloxycarbonyl; R_(H) is benzyl; R_(H2) is benzyl; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, tert-butyldiphenylsilyl;
 68. A disaccharide building block of General Formula XXI,

wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl; carbonate protecting groups; is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups, carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety; R_(M) is selected from the group consisting of ap-methoxyphenyl protecting group or other suitable oxidatively labile protecting group; a trityl group.
 69. The disaccharide building block of claim 67, wherein X₂ is selected from a a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate. R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(S5) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups tert-butyldiphenylsilyl; R_(M) is selected from the group consisting of a p-methoxyphenyl protecting group or other suitable oxidatively labile protecting group; a trityl group.
 70. The disaccharide building block of claim 68, wherein: X₂ is selected from thiomethyl, thiocresyl, trichloroacetimidoyl and tert-butyldiphenylsilyl, and the stereochemistry may be alpha or beta; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoate; or allyloxycarbonyl; R_(B1) is an azido function, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(H2) is benzyl; R_(H) is benzyl; R_(S5) is selected from the group consisting of 4-methoxyphenyl, 4-methoxybenzyl, benzoyl, tert-butyldiphenylsilyl, R_(M) is selected from the group consisting of a P-methoxyphenyl, 4-methoxybenzyl and a trityl group
 71. A disaccharide building block of General Formula XXII,

wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; A includes but is not limited to: H, Methoxy, Methyl; other suitable substituents will be known to those in the art.
 72. The disaccharide building block of claim 71, wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(B) is selected from the group consisting of an azido function, an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted 4 benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl arylacyl or alkylarylacyl protecting groups-carbonate protecting groups; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted 4-benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups, carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; A includes but is not limited to H, Methoxy, Methyl; other suitable substituents will be known to those in the art.
 73. The disaccharide building block of claim 71, wherein: X₂ is selected from a hydroxyl group; thiomethyl, thiocresyl, trichloroacetimidoyl or a ^(t)butyldiphenylsilyloxy; and the stereochemistry may be alpha or beta; R_(B) is an azido function, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(S3) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, allyloxycarbonyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, allyloxycarbonyl; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoate; or allyloxycarbonyl; A is H or Methoxy.
 74. A trisaccharide building block of General Formula XXIII,

wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups, alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl, or, benzyl and substituted benzyl groups; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; is selected from the group consisting of 4-methoxyphenyl, substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S4) and R_(B) may be combined to form a cyclic carbamate. R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate;
 75. The trisaccharide building block of claim 74, wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, chloro, fluoro, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(S5) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl;
 76. The trisaccharide building block of claim 74, wherein: X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, chloro, fluoro, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl; substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups including allyloxycarbonyl. R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(B1) is selected from the group consisting of an azido function, an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group; R_(S5) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-butyldiphenylsilyl.
 77. The trisaccharide building block of claim 74, wherein: R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; tert-Butyldiphenylsilyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate, R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; X₂ is selected from a thiomethyl, thioethyl, thiophenyl, thiocresyl, trichloroacetimidoyl, tert-butyldiphenylsilyloxy and the stereochemistry may be alpha or beta. R_(E1) is selected from the group consisting of methyl, allyl, benzyl; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoate; or allyloxycarbonyl; R_(H) is benzyl; R_(H2) is benzyl; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, benzoyl, tert-butyldiphenylsilyl; R_(B1) is selected from the group consisting of an azido function, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate.
 78. The trisaccharide building block of General Formula XXIV,

wherein: X₁ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) and R_(H1) are selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido, NH-Ac, NH-Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate, R_(S1) is selected from the group consisting of: 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups, alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group; or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups.
 79. The trisaccharide building block of claim 78, wherein: X₁ is selected from the group consisting of hydroxy, alkoxy, aryloxy, benzyloxy; substituted benzyloxy; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) and R_(H1) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido, or other such suitable protected amino functions or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1), R_(S2), R_(S3) and R_(S4) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; substituted benzyl groups, alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, benzoyl, arylacyl or alkylarylacyl protecting groups, carbonate protecting groups; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E2) is selected from methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(B) is selected from an azido function, an amine; an NH-Dde or NH-DTPM group; additionally R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group, a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-Ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group.
 80. The trisaccharide building block of claim 78, wherein, X₁ is selected from the group consisting of hydroxy, methoxy, thiomethyl, thioethyl, thiocresyl, trichloroacetimidoyl, a ^(t)butyldiphenylsilyloxy; a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) and R_(H1) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is an azido, or R_(H1) and R_(A) can combine together to form a cyclic carbamate, R_(S1), R_(S2), R_(S3) and R_(S4) are independently selected from: methoxyphenyl; benzoyl or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E2) is selected from the group consisting of methyl, allyl or, benzyl and substituted benzyl groups, R_(B) is an azido function, or R_(S4) and R_(B) can combine together to form a cyclic carbamate;
 81. The tetrasaccharide building block of General Formula XXV,

wherein, X₁ is selected from the group consisting of hydroxy, alkenyloxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy; thioalkyl, thioaryl, halogen, imidoyl, phosphate and related phosphate ester type leaving groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid or other such group suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H1) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, an amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido, NH-Ac, NH-Allyloxycarbonyl; or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S2) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S3) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate, R_(E1) a is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(E2) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl, C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups, R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group; or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups, carbonate protecting groups; benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl, R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting groups; allyloxycarbonyl, allyl ether, carbonate type protecting groups.
 82. The tetrasaccharide building block of claim 81, wherein, X₁ is selected from the group consisting of hydroxy, alkoxy, aryloxy, benzyloxy, substituted benzyloxy, thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta; R_(H) and R_(H1) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is selected from the group consisting of an azido function, and amine; an NH-Dde, NH-DTPM, NH-Fmoc, NH-Boc, NH-Troc, N-phthalimido; or other such suitable protected amino functions or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1), R_(S2), R_(S3) and R_(S4) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, benzoyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(B) is selected from an azido function, an amine; an NH-Dde or NH-DTPM group; additionally R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(L) is selected from a H atom; a levulinoyl, chloroacetyl, 4-acetoxybenzoyl, 4-acetamidobenzoyl, 4-azidobenzoyl, or other substituted benzoyl type protecting group; a benzyl group, a 4-acetoxybenzyl, 4-acetamidobenzyl or other such suitable substituted benzyl type protecting group; γ-aminobutyryl, 4-N-[1-(4,4-dimethyl-2,6-dioxocyclohex-1-Ylidene)ethylamino]-butyryl, 4-N-[1-(1,3-dimethyl-2,4,6(1H,3H,5H)-trioxopyrimidin-5-ylidene)methylamino]-butyryl, 4-N-Alloc-butyryl, 4-N-Fmoc-butyryl, 4-N-Boc-butyryl type protecting group.
 83. The tetrasaccharide building block of claim 81, wherein: X₁ is selected from the group consisting of hydroxy, methoxy, thiomethyl, thioethyl, thiocresyl, trichloroacetimidoyl, a ^(t)butyldiphenylsilyloxy a lipoaminoacid suitable for conjugation to delivery systems or solid supports; and the stereochemistry may be alpha or beta, R_(H) and R_(H1) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(A) is an azido function, or R_(H1) and R_(A) can combine together to form a cyclic carbamate; R_(S1), R_(S2), R_(S3), and R_(S4) are independently selected from: the group consisting of 4-methoxyphenyl; 4-methoxybenzyl; benzoyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate, R_(E1) and R_(E2) are independently selected from methyl, allyl or, benzyl and substituted benzyl groups; R_(B) is an azido function, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(B) is an azido function, or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(L) is selected from a H atom; a levulinoyl.
 84. The tetrasaccharide building block of General Formula XXVI,

wherein: R_(H) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl; R_(H2) is selected from the group consisting of benzyl or substituted benzyl protecting group, allyl, allyloxycarbonyl, or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(S2) is selected from the group consisting of: 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxymethyl, benzyloxymethyl; R_(S3) is selected from the group consisting of 4-methoxyphenyl, substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; R_(S4) is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups, carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(S5) is selected from the group consisting of 4-methoxyphenyl; 4-methoxybenzyl, substituted benzyl groups; alkylacyl, benzoyl, arylacyl or alkylarylacyl, and substituted alkylacyl, 4-chlorobenzoyl, arylacyl or alkylarylacyl protecting groups; allyloxycarbonyl, ethoxycarbonyl, tertbutoxycarbonyl, carbonate protecting groups; is selected from the group consisting of 4-methoxyphenyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group allyl, methoxymethyl, methoxyethyl, benzyloxymethyl; or R_(S5) and R_(H) can be combined to form a cyclic acetal or ketal moiety, R_(E1) is selected from the group consisting of methyl, C₂-C₅ alkyl; substituted alkyl; C₃-C₅ alkenyl; or, benzyl and substituted benzyl groups; R_(B) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group; or R_(S4) and R_(B) can combine together to form a cyclic carbamate; R_(B1) is selected from the group consisting of an azido function, an amine; an NH-Dde or NH-DTPM group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(P1) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl, and alkylarylacyl protecting groups; and carbonate protecting groups; R_(P2) is selected from the group consisting of 4-methoxyphenyl; benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl and alkylarylacyl protecting groups; carbonate protecting groups, silyl protecting groups, carbamate protecting groups, C₃-C₅ alkenyl, X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta.
 85. The tetrasaccharide building block and claim 84, wherein: R_(H) and R_(H2) are independently selected from a benzyl or substituted benzyl protecting group, R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; substituted benzyl groups; alkylacyl, arylacyl or alkylarylacyl, and substituted alkylacyl, benzoyl, arylacyl or alkylarylacyl protecting groups; carbonate protecting groups; or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, C₂-C₅ alkyl; substituted alkyl; or, benzyl and substituted benzyl groups; R_(B) and R_(B1) are independently selected from an azido function; an amine; an NH-Dde or NH-DTPM group; additionally R_(S4) and R_(B) or R_(H2) and R_(B1) independently can combine together to form a cyclic carbamate; R_(P1) and R_(P2) are independently selected from benzyl, substituted benzyl groups; alkylacyl, arylacyl and alkylarylacyl, or substituted alkylacyl, arylacyl, and alkylarylacyl protecting groups; and carbonate protecting groups; X₂ is selected from a hydroxyl group; thioalkyl, thioaryl, halogen, trichloroacetimidoyl, phosphate and related phosphate ester type leaving groups, a ^(t)butyldiphenylsilyloxy or other such substituted silyloxy protecting group; and the stereochemistry may be alpha or beta.
 86. The tetrasaccharide building block of claim 84, wherein: R_(H) and R_(H2) are independently selected from a benzyl or substituted benzyl protecting group, or R_(H2) and R_(B1) can combine together to form a cyclic carbamate; R_(S2), R_(S3), R_(S4) and R_(S5) are independently selected from: 4-methoxyphenyl; 4-methoxybenzyl; benzoyl, or R_(S4) and R_(B) may be combined to form a cyclic carbamate; R_(E1) and R_(E2) are independently selected from methyl, allyl or, benzyl and substituted benzyl groups; R_(B) and R_(B1) are independently an azido function, or (R_(S4) and R_(B)) or (R_(H2) and R_(B1)) independently can combine together to form a cyclic carbamate; R_(P1) is benzyl; R_(P2) is selected from the group consisting of benzyl; benzoyl, allyloxycarbonyl; X₂ is selected from a hydroxyl group; thiomethyl, thiocresyl trichloroacetimidoyl, or ^(t)butyldiphenylsilyloxy; and the stereochemistry may be alpha or beta.
 87. A pentasaccharide of the formula,

formed by the deprotection of the pentasaccharide of claim
 1. 88. A pharmaceutical preparation comprising a pharmaceutically effective amount of the pentasaccharide of claim 87 together with a pharmaceutical carrier.
 89. A pentasaccharide of the formula

formed by deprotection of the pentasaccharide of claim
 4. 